Cloning and characterization of elastase structural gene from Pseudomonas aeruginosa IFO 3455.
Cloning and characterization of elastase structural gene from Pseudomonas aeruginosa IFO 3455.
复制标题
铜绿假单胞菌 IFO 3455 弹性蛋白酶结构基因的克隆和表征。
DOI：
10.1016/s0006-291x(88)80400-5
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发表时间：
1988
影响因子：
3.1
通讯作者：
K. Morihara
中科院分区：
文献类型：
--
作者：
S. Yamamoto;J. Fukushima;Y. Atsumi;H. Takeuchi;S. Kawamoto;K. Okuda;K. Morihara
从铜绿假单胞菌IFC3455中克隆出一条8.3kb的DNA片段。这个含有Escherichiacclone片段的pEL2产生了高水平的弹性蛋白酶活性。将较小的EcoRI-KpnI片段亚克隆到pUC118中。将该表达载体转化到大肠杆菌HB101中。在同一细菌中还构建了缺失突变克隆。对这些缺失突变体进行了弹性蛋白酶活性检测，结果表明，弹性蛋白酶基因全长为1.0-1.3kb。DNA测序分析表明，该DNA片段含有编码弹性蛋白酶蛋白N端氨基酸序列的DNA序列。
An 8.3 Kb DNA fragment was cloned fromPseudomonas aeruginosaIFC 3455. This fragment-containingEscherichiaclone, pEL2, produced a high level of elastase activity. A smallerEcoRI-KpnIfragment was subcloned into pUC118 andE. coliHB101 was transformed with the plasmid. A deletion mutant clone was also constructed in the same bacteria. These deletion mutants were tested for elastase activity and it became clear that the full length of the elastase gene was 1.0–1.3 Kb. DNA sequencing analysis revealed that this DNA fragment contains the DNA sequence coding N-terminal amino acid sequence of the elastase protein.
影响因子：
3.5
作者：
HENIKOFF, S
通讯作者：
HENIKOFF, S