Characterization of keratinocyte plasminogen activator inhibitors and demonstration of the prevention of pemphigus IgG-induced acantholysis by a purified plasminogen activator inhibitor.
Characterization of keratinocyte plasminogen activator inhibitors and demonstration of the prevention of pemphigus IgG-induced acantholysis by a purified plasminogen activator inhibitor.
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角质形成细胞纤溶酶原激活剂抑制剂的表征以及通过纯化的纤溶酶原激活剂抑制剂预防天疱疮 IgG 诱导的棘层松解的演示。
DOI：
10.1111/1523-1747.ep12277087
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Jensen,PJ
中科院分区：
文献类型：
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作者：
Hashimoto,K;Wun,TC;Baird,J;Lazarus,GS;Jensen,PJ
为了研究皮肤纤溶酶原激活物（PA）可能被调节的机制，我们测试了培养的角质形成细胞中PA抑制剂的存在。利用35ss -蛋氨酸结合特异性抗体沉淀的生物合成标记实验，我们发现培养的人角质形成细胞可以合成和分泌PA抑制剂I和PA抑制剂2。PA抑制剂1在条件培养基中以失活形式存在，但可以用反相自显影法检测。PA抑制剂2通过其与125i - upa形成配合物的能力来检测。皮肤器官培养实验表明，纯化的人胎盘PA抑制剂2能够预防天疱疮IgG引起的棘皮溶解性改变，表明皮肤PA抑制剂2具有潜在的治疗相关性。
To investigate the mechanisms by which cutaneous plasminogen activator (PA) may be regulated, we have tested cultured keratinocytes for the presence of PA inhibitors. Using biosynthetic labeling experiments with35SS-methionine in conjunction with specific antibody precipitation, we have shown that human keratinocytes in culture synthesized and secreted both PA inhibitor I and PA inhibitor 2. PA inhibitor 1 was present in conditioned media in the inactive form, but it could be detected with reverse phase autography. PA inhibitor 2 was detected by its ability to form complexes with125I-uPA. Potential therapeutic relevance for cutaneous PA inhibitor 2 was suggested in skin organ culture experiments which demonstrated that purified PA inhibitor 2 from human placenta was able to prevent the acantholytic changes induced by pemphigus IgG.