Negative signaling contributes to T-cell anergy in trauma patients
Negative signaling contributes to T-cell anergy in trauma patients
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DOI：
10.1097/01.ccm.0000256847.61085.a5
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发表时间：
2007-03-01
影响因子：
8.8
通讯作者：
Miller-Graziano, Carol
中科院分区：
文献类型：
--
作者：
Bandyopadhyay, Gautam;De, Asit;Miller-Graziano, Carol
目的：损伤后T淋巴细胞免疫麻痹或无反应性的维持可能是由于在T细胞受体活化期间未能活化共刺激受体和/或由于竞争性升高的共阻遏物受体的慢性刺激。我们的目的是评估创伤后T淋巴细胞表面辅阻遏物受体表达的升高是否与免疫抑制的淋巴细胞应答相关，并与抑制性信号转导分子的增加和活化性信号转导分子的减少相对应。大学创伤重症监护室和研究实验室。患者：61例严重热创伤和机械创伤患者。干预：无。测量和主要结果。，分别检测创伤患者和对照组外周血T细胞的阴性和阳性共刺激受体表达。这些受体的表达水平进行了比较（流式细胞术）两组之间的抑制和激活信号转导分子和增殖能力的T细胞水平。患者的增殖低反应性（无反应性）T细胞增加了新型抑制性受体（辅阻遏物）PD-1的表达（p
Objective: Maintenance of postinjury T-lymphocyte immune paralysis or anergy could result from failure to activate costimulatory receptors during T-cell receptor activation and/or from chronic stimulation of a competing set of elevated corepressor receptors. Our objective was to assess whether elevated post-trauma T-lymphocyte surface expression of corepressor receptors was associated with immunodepressed lymphocyte responses and corresponded to increased inhibitory and decreased activating signal transduction molecules.Design: Prospective observational study.Setting., University trauma intensive care unit and research laboratory.Patients: Sixty-one severe thermal and mechanical trauma patients.Interventions: None.Measurements and Main Results., Isolated trauma patients' and controls' peripheral blood T cells were assayed for negative and positive costimulation receptor expression. These receptor expression levels were compared (flow cytometry) between the two groups and correlated to T-cell levels of inhibitory and activating signal transduction molecules and proliferation capacity. Patients' proliferation hyporesponsive (anergic) T cells had increased expression of novel inhibitory receptors (corepressors) PD-1 (p