Linkages between Epithelial Microbiota and Host Transcriptome in the Ileum during High-Grain Challenges: Implications for Gut Homeostasis in Goats
Linkages between Epithelial Microbiota and Host Transcriptome in the Ileum during High-Grain Challenges: Implications for Gut Homeostasis in Goats
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高谷物挑战期间回肠上皮微生物群与宿主转录组之间的联系：对山羊肠道稳态的影响
DOI：
10.1021/acs.jafc.8b05591
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发表时间：
2019-01-09
影响因子：
6.1
通讯作者：
Tan, Zhiliang
中科院分区：
文献类型：
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作者：
Jiao, Jinzhen;Zhang, Xiaoli;Tan, Zhiliang
高谷物 (HG) 饮食可导致瘤胃亚急性酸中毒，这不利于肠道健康，并可能导致生产力下降。本研究调查了饲喂 HG 日粮（浓缩物/干草，90:10）和对照日粮（浓缩物/干草，55:45）的山羊的回肠上皮微生物群及其与宿主上皮功能的关系，旨在阐明回肠适应亚急性酸中毒的机制。 HG 挑战增加了回肠挥发性脂肪酸浓度 (p = 0.030)，并通过增加 (FDR < 0.05) 活性碳水化合物和蛋白质降解剂 Synergistetes、Prevotella、Fibrobacter、Clostridium、Treponema 和未分类 Ruminococcaceae 的相对丰度 (20.1-、6.3-、16.8-) 来改变回肠上皮微生物群。分别是 8.5 倍、19.9 倍和 7.1 倍。然而，HG 饮食倾向于降低 (FDR < 0.10) Candidatus Arthromitus 的相对丰度（38.8 +/- 36.1 对比 2.1 +/- 3.1）。使用 PICRUSt 推断的微生物功能潜力表明，HG 挑战提高了与氨基酸、聚糖、辅因子和维生素代谢相关的途径的丰度，而减少了与信号转导、外源生物降解和代谢相关的途径。此外，在 HG 山羊的回肠上皮中，转录组分析发现参与碳水化合物、脂质、蛋白质、维生素和促炎细胞因子途径代谢的候选基因增加 (FDR < 0.10)，同时下调编码抗菌剂和补体的基因 (FDR < 0.05)。总的来说，HG 挑战改变了回肠微生物群落的结构和功能潜力，并影响了山羊回肠中的宿主反应，增加了常量和微量营养素的代谢活动，同时增加了肠道炎症的风险。
A high-grain (HG) diet can result in ruminal subacute acidosis, which is detrimental to gut health and can lead to decreased productivity. This study investigated the ileal epithelial microbiota and its relationship with host epithelial function in goats fed a HG diet (concentrate/hay, 90:10) and a control diet (concentrate/hay, 55:45), aiming to elucidate the mechanisms involved in ileal adaptation to subacute acidosis. The HG challenge increased the ileal volatile fatty acid concentration (p = 0.030) and altered the ileal epithelial microbiota by increasing (FDR < 0.05) relative abundances of active carbohydrate and protein degraders Synergistetes, Prevotella, Fibrobacter, Clostridium, Treponema, and unclassified Ruminococcaceae by 20.1-, 6.3-, 16.8-, 8.5-, 19.9-, and 7.1-fold, respectively. However, the HG diet tended to reduce (FDR < 0.10) the relative abundance of Candidatus Arthromitus (38.8 +/- 36.1 versus 2.1 +/- 3.1). Microbial functional potentials inferred using PICRUSt indicated that the HG challenge elevated abundances of pathways associated with metabolism of amino acid, glycan, cofactors, and vitamins, whereras it decreased pathways associated with signal transduction, xenobiotic biodegradation, and metabolism. Additionally, in the ileal epithelium of HG goats, transcriptome analysis identified the increment (FDR < 0.10) of candidate genes involved in metabolism of carbohydrates, lipids, proteins, vitamins, and the proinflammatory cytokine pathway, while downregulating genes encoding antimicrobials and complements (FDR < 0.05). Collectively, the HG challenge shifted the structure and functional potentials of the ileal microbial community and affected the host responses in the ileum of goats toward increased metabolic activities of macro- and micronutrients, together with an increased risk of gut inflammation.