beta-Cryptoxanthin stimulates bone formation and inhibits bone resorption in tissue culture in vitro.
beta-Cryptoxanthin stimulates bone formation and inhibits bone resorption in tissue culture in vitro.
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β-Cryptoxanthin 在体外组织培养中刺激骨形成并抑制骨吸收。
DOI：
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发表时间：
2004
影响因子：
4.3
通讯作者：
S. Uchiyama
中科院分区：
文献类型：
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作者：
M. Yamaguchi;S. Uchiyama
大量存在于水果中的β-隐黄质对骨代谢的影响迄今尚未阐明。在体外组织培养中研究了β-隐黄素对骨形成和骨吸收的影响。大鼠股骨骨干（皮质骨）和干骺端（骨小梁）组织培养48小时的Dulbecco的改良Eagle培养基（高葡萄糖，4.5%）补充抗生素和牛血清白蛋白。实验培养物含有10（-8）-10（-5）M β-隐黄素。β-隐黄素（10（-6）或10（-5）M）的存在导致骨干和干骺端组织中钙含量、碱性磷酸酶活性和脱氧核糖核酸（DNA）含量显著增加。这些增加在放线菌酮（10（-6）M），蛋白质合成的抑制剂的存在下被完全阻止。β-胡萝卜素（10（-6）或10（-5）M）或黄嘌呤（10（-6）或10（-5）M）对骨干和干骺端的钙含量没有影响。骨吸收因子甲状旁腺激素（1-34）（PTH; 10（-7）M）或前列腺素E2（PGE 2; 10（-5）M）导致骨干和干骺端组织中钙含量显著降低。β-隐黄素（10（-8）~ 10（-6）M）可完全抑制PTH或PGE_2引起的骨钙含量的降低。此外，β-隐黄素（10（-8）-10（-6）M）完全抑制PTH（10（-7）M）或PGE（10（-5）M）诱导的骨干和干骺端组织中葡萄糖消耗和乳酸产生的增加。β-隐黄质（10（-7）M）对PTH（10（-7）M）或PGE 2（10（-5）M）刺激的骨干钙含量降低的抑制作用在10（-3）M钒酸盐（一种蛋白酪氨酸磷酸酶抑制剂）存在下被显著阻止。钒酸盐（10（-3）M）对对照骨组织中的钙含量和乳酸产生没有显著影响。本研究表明，β-隐黄素在体外组织培养中对骨形成具有直接的刺激作用，对骨吸收具有抑制作用。
The effect of beta-cryptoxanthin, which is greatly present in fruits, has not been clarified so far on bone metabolism. The effect of beta-cryptoxanthin on bone formation and bone resorption was investigated in tissue culture in vitro. Rat femoral-diaphyseal (cortical bone) and -metaphyseal (trabecular bone) tissues were cultured for 48 h in Dulbecco's modified Eagle's medium (high glucose, 4.5%) supplemented with antibiotics and bovine serum albumin. The experimental cultures contained 10(-8)-10(-5) M beta-cryptoxanthin. The presence of beta-cryptoxanthin (10(-6) or 10(-5) M) caused a significant increase in calcium content, alkaline phosphatase activity and deoxyribonucleic acid (DNA) content in the diaphyseal and metaphyseal tissues. These increases were completely prevented in the presence of cycloheximide (10(-6) M), an inhibitor of protein synthesis. beta-Carotene (10(-6) or 10(-5) M) or xantine (10(-6) or 10(-5) M) had no effect on the diaphyseal and metaphyseal calcium contents. The bone-resorbing factors parathyroid hormone (1-34) (PTH; 10(-7) M) or prostaglandin E2 (PGE2; 10(-5) M) caused a significant decrease in calcium content in the diaphyseal and metaphyseal tissues. The decrease in bone calcium content induced by PTH or PGE2 was completely inhibited by beta-cryptoxanthin (10(-8)-10(-6) M). In addition, beta-cryptoxanthin (10(-8)-10(-6) M) completely inhibited the PTH (10(-7) M)- or PGE, (10(-5) M)-induced increase in medium glucose consumption and lactic acid production by diaphyseal and metaphyseal tissues. The inhibitory effect of beta-cryptoxanthin (10(-7) M) on PTH (10(-7) M)- or PGE2 (10(-5) M)-stimulated decrease in the diaphyseal calcium content was significantly prevented in the presence of 10(-3) M vanadate, an inhibitor of protein tyrosine phosphatase. Vanadate (10(-3) M) did not have a significant effect on calcium content and lactic acid production in control bone tissues. The present study demonstrates that beta-cryptoxanthin has a direct stimulatory effect on bone formation and an inhibitory effect on bone resorption in tissue culture in vitro.