Mitochondrial DNA variability in natural populations of Hawaiian Drosophila. I. Methods and levels of variability in D. silvestris and D. heteroneura populations.
Mitochondrial DNA variability in natural populations of Hawaiian Drosophila. I. Methods and levels of variability in D. silvestris and D. heteroneura populations.
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夏威夷果蝇自然群体中线粒体 DNA 的变异性。
DOI：
10.1038/hdy.1986.11
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发表时间：
1986
期刊：
影响因子：
3.8
通讯作者：
Templeton,AR
中科院分区：
文献类型：
--
作者：
DeSalle,R;Giddings,LV;Templeton,AR
我们描述的技术，线粒体DNA（mtDNA）限制性位点的信息，可以获得多达16个不同的限制性内切酶对个别夏威夷果蝇，特别是D。silvestris和D.异脉类我们构建了来自夏威夷岛7个主要采集点的48个野生捕获的两个物种基因组的mtDNA限制性酶切位点图谱。一般来说，D的变异水平较高。silvestris（Ewens等人的p，1981= 0· 0486），D.异神经症（p= 0· 0327）。使用Nei的G ST的人口细分的措施表明，约50- 60%的观察到的变异是由于种群间的细分。因此，一个物种内的种群表现出低得多的变异性，但有些种群的个体与同一地点的其他同种个体差异很大。我们回顾了两种可能的机制，可以解释这些不同的个人，杂交和随机分支过程的影响的存在。
We describe techniques by which mitochondrial DNA (mtDNA) restriction site information can be obtained for up to 16 different restriction endonucleases on individual Hawaiian Drosophila, in particular D. silvestris and D. heteroneura. We have constructed mtDNA restriction site maps for a total of forty-eight wild caught genomes of both species, from seven major collecting sites on the island of Hawaii. Levels of variability are, in general, high in D. silvestris (p of Ewens et al., 1981= 0· 0486) and lower in D. heteroneura (p= 0· 0327). Measures of population subdivision using Nei's G st indicate that about 50–60 per cent of the observed variability is due to interdemic subdivision. In accordance, populations within a species show a much lower level of variability, however some populations harbour individuals that are very divergent from the rest of their conspecifics at the same locality. We review two possible mechanisms that could explain the presence of these divergent individuals, hybridisation and the effects of stochastic branching processes.