Intraepithelial infiltration of eosinophils and their contribution to the elimination of adult intestinal nematode, Strongyloides venezuelensis in mice
Intraepithelial infiltration of eosinophils and their contribution to the elimination of adult intestinal nematode, Strongyloides venezuelensis in mice
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DOI：
10.1016/s1383-5769(02)00086-7
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发表时间：
2003-03-01
影响因子：
1.9
通讯作者：
Ohta, N
中科院分区：
文献类型：
--
作者：
El-Malky, M;Maruyama, H;Ohta, N
用白介素5转基因小鼠检测嗜酸性粒细胞对肠道粘膜内松材线虫成虫的攻击能力。在IL-5转基因小鼠中，皮下接种的感染幼虫大部分在迁移过程中被杀死，只有少数蠕虫可以到达小肠。将相同数量的成虫移植到IL-5转基因小鼠和对照小鼠的小肠内时，IL-5转基因小鼠的粪卵产量和从肠道中回收的成虫数量明显减少。在IL-5转基因小鼠的肠粘膜中，甚至在成虫植入之前，固有层中就已经出现了大量的嗜酸性粒细胞。在植入后24小时，嗜酸性粒细胞数量显著增加，到第3天时增加了两倍，而野生型小鼠的粘膜嗜酸性粒细胞数量仍然很低。最值得注意的是，在IL-5转基因小鼠中，嗜酸性粒细胞渗入肠道上皮细胞并包围成虫，这在野生型对照小鼠中从未见过。然而，IL-5转基因小鼠完全清除植入的成虫所需的时间与正常小鼠相同。IL-5转基因小鼠在成虫植入前粪便中特异性IgA和总IgA含量较高，成虫植入后迅速下降。目前的研究表明，嗜酸性粒细胞能够攻击肠道上皮中的成虫，可能与分泌的IgA一起攻击，尽管它们不足以完全驱除线虫。(C)2002爱思唯尔科学爱尔兰有限公司。保留所有权利。
Eosinophils were examined for the capacity of attacking Strongyloides venezuelensis adult worms in the intestinal mucosa by using interleukin (IL)-5 transgenic mice. In IL-5 transgenic mice, most of the subcutaneously inoculated infective larvae were killed during migration, and only a few worms could reach the small intestine. When the same number of adult worms were surgically implanted in the small intestine of IL-5 transgenic and control mice, fecal egg output as well as the number of adult worms recovered from the intestine was significantly lower in IL-5 transgenic mice. In the intestinal mucosa of IL-5 transgenic mice, large number of eosinophils was present in the lamina propria even before adult worm implantation. The number of eosinophils increased significantly as early as 24 h after implantation and tripled by day 3, whereas mucosal eosinophilia remained low in wild type mice. Most notably, eosinophils infiltrated into the intestinal epithelium and surrounded adult worms in IL-5 transgenic mice, which was never seen in wild-type control mice. However, IL-5 transgenic mice required the same period as normal mice to completely expel implanted adult worms. The amount of specific IgA as well as total IgA in the stool was high in IL-5 transgenic mice before adult worm implantation, and dropped rapidly after adult worm implantation. The present study suggests that eosinophils are capable of attacking adult nematodes in the intestinal epithelia, probably in conjunction with secretory IgA, although they are not enough for the complete worm expulsion. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.