VIABILITY OF HUMAN CORNEAL ENDOTHELIUM FOLLOWING OPTISOL-GS STORAGE
VIABILITY OF HUMAN CORNEAL ENDOTHELIUM FOLLOWING OPTISOL-GS STORAGE
复制标题
DOI：
10.1001/archopht.1995.01100060131047
复制
发表时间：
1995-06-01
影响因子：
--
通讯作者：
EDELHAUSER, HF
中科院分区：
文献类型：
--
作者：
MEANS, TL;GEROSKI, DH;EDELHAUSER, HF
目的：为了评估在玻璃瓶和观察室（Alcon）中储存较长时间的人角膜的内皮活力，并比较Optisol-GS储存的角膜与从湿室储存的globes.Methods切下的角膜的内皮活力：使用两种染色技术评估内皮活力。用台盼蓝与茜素红S联合染色或钙黄绿素AM-乙锭同型二聚体染色保存的角膜内皮细胞。两种技术被用来确定哪种方法是一个更敏感的指标细胞毒性changes.Results：角膜储存在Optisol-GS 4至21天有一个率（平均值+/- SE）的内皮细胞损伤的0.57% +/- 0.30%，每天在小瓶和0.69% +/- 0.27%，在室内。在4至21天的储存间隔后，Optisol-GS内皮细胞的活力平均降低9.5%至16%。湿房眼的内皮细胞在2 ~ 5天后活力下降44%~ 59%。24小时后，湿房眼的角膜内皮细胞活力下降小于15%。储存35至56天的Optisol-GS角膜内皮活力降低超过50%。67天后，95%至100%的内皮细胞活力losed.Conclusions：角膜保存在Optisol-GS通过21天，在4摄氏度保持高百分比的活内皮细胞。保存在玻璃瓶或观察室（Alcon）中的角膜之间的内皮活力无显著差异。湿室储存的角膜在2天后和Optisol-GS中储存的角膜在35天后发生50%的内皮活力损失。
Objectives: To evaluate endothelial viability of human corneas stored in glass vials and in viewing chambers (Alcon) for extended periods, and to compare endothelial viability of Optisol-GS-stored corneas with corneas excised from moist chamber-stored globes.Methods: Endothelial viability was assessed using two staining techniques. Endothelium from stored corneas was stained with trypan blue combined with alizarin red S or stained with calcein AM-ethidium homodimer. Both techniques were used to determine which method is a more sensitive indicator of cytotoxic change.Results: Corneas stored 4 to 21 days in Optisol-GS had a rate (mean +/- SE) of endothelial cell damage of 0.57% +/- 0.30% per day in vials and 0.69% +/- 0.27% in chambers. After storage intervals from 4 to 21 days, the Optisol-GS endothelium had an average decrease in viability of 9.5% to 16%. The endothelium of moist chamber eyes had a 44% to 59% decrease in viability after 2 to 5 days. After 24 hours, corneal endothelium of moist chamber eyes had less than 15% decrease in viability. Optisol-GS corneas stored for 35 to 56 days had greater than 50% decrease in endothelial viability. After 67 days, 95% to 100% of endothelial viability was lost.Conclusions: Corneas stored in Optisol-GS through 21 days at 4 degrees C maintain a high percentage of viable endothelial cells. There was no significant difference of endothelial viability between corneas stored in glass vials or in viewing chambers (Alcon). A 50% loss of endothelial viability occurred in moist chamber-stored corneas after 2 days and by 35 days in corneas stored in Optisol-GS.