Effects of the Crotalus durissus terrificus Snake Venom on Hepatic Metabolism and Oxidative Stress
Effects of the Crotalus durissus terrificus Snake Venom on Hepatic Metabolism and Oxidative Stress
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DOI：
10.1002/jbt.20376
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发表时间：
2011-05-01
影响因子：
3.6
通讯作者：
Acco, Alexandra
中科院分区：
文献类型：
--
作者：
da Silva, Jonas Golart;Soley, Bruna da Silva;Acco, Alexandra
蛇毒成分复杂，主要以毒素和酶为代表，因此表现出不同的作用机制。这项工作旨在研究响尾蛇 (Cdt) 毒液对肝脏的影响。 Wistar 大鼠腹膜内接种生理盐水（对照）或 Cdt 毒液。 3、4或6小时后，分析以下参数：（a）肝功能，（b）氧化应激参数，和（c）离体灌注肝脏中丙氨酸的代谢。接种300μg.kg(-1)Cdt毒液后，大鼠血浆丙氨酸转氨酶、天冬氨酸转氨酶、肝谷胱甘肽S-转移酶、过氧化氢酶活性显着升高。毒液剂量为100、200和300μg.kg(-1)时，肝脏脂质过氧化作用显着增加，而谷胱甘肽S-转移酶没有变化。当丙氨酸为代谢底物时，接种1500μg.kg(-1)毒液的大鼠的灌注肝脏显示乳酸、丙酮酸和氨的产生增加。这些结果表明，Cdt 毒液可以在肝细胞中产生多种变化。这些变化的原因可能与氧化还原稳态的不平衡有关，但也与中毒生物体的特定需求有关，例如，响应科里循环活性增加而增加乳酸和丙酮酸的供应。 C (C) 2010 Wiley periodicals, Inc. J BiochemMol Toxicol 25: 195-203, 2011；请访问 wileyonlinelibrary.com 在线查看本文。 DOI 10:1002/jbt.20376
Snake venoms present different action mechanisms because of their complex composition, represented mainly by toxins and enzymes. This work aimed to investigate the effects of the Crotalus durissus terrificus (Cdt) venom in the liver. Wistar rats were inoculated intraperitoneally with saline (control) or Cdt venom. After 3, 4, or 6 h, the following parameters were analyzed: (a) hepatic function, (b) oxidative stress parameters, and (c) the metabolism of alanine in the isolated perfused liver. Plasma activities of alanine aminotransferase and aspartate aminotransferase and hepatic glutathione S-transferase and catalase presented significant elevation in rats inoculated with 300 mu g.kg(-1) Cdt venom. Liver lipoperoxidation was enormously increased by venom doses of 100, 200, and 300 mu g.kg(-1), whereas glutathione S-transferase was not changed. Perfused livers from rats inoculated with 1500 mu g.kg(-1) venom showed increased production of lactate, pyruvate, and ammonia when alanine was the metabolic substrate. These results demonstrate that the Cdt venom can produce several changes in hepatocytes. The causes of the changes are possibly related to the disequilibrium in the redox homeostasis but also to specific needs of the poisoned organism, for example, an increased supply of lactate and pyruvate in response to an increased activity of the Cori cycle. C (C) 2010 Wiley Periodicals, Inc. J BiochemMol Toxicol 25: 195-203, 2011; View this article online at wileyonlinelibrary.com. DOI 10:1002/jbt.20376