Effect of CPP-ACP paste on tooth mineralization: an FE-SEM study.
Effect of CPP-ACP paste on tooth mineralization: an FE-SEM study.
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DOI：
10.2334/josnusd.49.115
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发表时间：
2007-06-01
影响因子：
1.9
通讯作者：
Miyazaki, Masashi
中科院分区：
文献类型：
--
作者：
Oshiro, Maki;Yamaguchi, Kanako;Miyazaki, Masashi
牛奶和奶制品（例如奶酪）已在人类和动物模型中显示出抗龋齿特性。 CPP-ACP 通过抑制脱矿、增强再矿化或可能两者的组合来显示抗龋齿作用。本研究的目的是通过使用 FE-SEM 观察处理过的牙齿表面来评估 CPP-ACP 糊剂对脱矿质的影响。通过将牛牙齿的牙釉质和牙本质切成块来制备标本。将一些标本保存在 0.1 M 乳酸缓冲溶液中 10 分钟，然后保存在人工唾液中（阴性对照）。其余标本保存在 10 倍稀释的 CPP-ACP 糊剂溶液或不含 CPP-ACP 的安慰剂糊剂溶液中 10 分钟，然后在脱盐溶液（pH = 4.75，Ca）中浸泡 10 分钟，每天两次，然后保存在人工唾液中。标本处理3、7、21和28天后，将其固定在含2.5%戊二醛的二甲胂酸盐缓冲溶液中，在递增等级的叔丁醇中脱水，然后转移至临界点干燥器。在真空蒸发器中在表面镀上一层金薄膜，并在场发射扫描电子显微镜（FE-SEM）下观察。 SEM观察揭示了不同储存条件带来的不同形态特征。在对照和阴性对照标本中，随着测试时间的延长，牙釉质和牙本质表面的脱矿质更加明显。另一方面，用 CPP-ACP 糊剂处理的牙釉质和牙本质标本显示出其形态特征的轻微变化。从牙釉质和牙本质表面的形态观察，可以认为CPP-ACP糊剂可以防止牙齿结构的脱矿。
Milk and milk products, such as cheese, have been shown to exhibit anticariogenic properties in human and animal models. CPP-ACP shows an anti-caries effect by suppressing demineralization, enhancing remineralization, or possibly a combination of both. The purpose of this study was to evaluate the effect of CPP-ACP paste on demineralization by observing the treated tooth surface using an FE-SEM. The specimens were prepared by cutting enamel and dentin of bovine teeth into blocks. A few specimens were stored in 0.1 M lactic acid buffer solution for 10 min and then in artificial saliva (negative control). The remaining specimens were stored in a 10 times-diluted solution of CPP-ACP paste or a placebo paste containing no CPP-ACP for 10 min, followed by 10 min immersion in a demineralizing solution (pH = 4.75, Ca) twice a day before storage in artificial saliva. After treatment of the specimens for 3, 7, 21 and 28 days, they were fixed in 2.5% glutaraldehyde in cacodylate buffer solution, dehydrated in ascending grades of tert-butyl alcohol, and then transferred to a critical-point dryer. The surfaces were coated with a thin film of Au in a vacuum evaporator, and were observed under field emission-scanning electron microscopy (FE-SEM). The SEM observations revealed different morphological features brought about by the various storage conditions. Demineralization of the enamel and dentin surfaces was more pronounced with the longer test period in the control and negative control specimens. On the other hand, enamel and dentin specimens treated with CPP-ACP paste revealed slight changes in their morphological features. From the morphological observations of the enamel and dentin surfaces, it could be considered that the CPP-ACP paste might prevent demineralization of the tooth structure.