A bulged stem-loop structure in the 3' untranslated region of the genome of the coronavirus mouse hepatitis virus is essential for replication
A bulged stem-loop structure in the 3' untranslated region of the genome of the coronavirus mouse hepatitis virus is essential for replication
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DOI：
10.1128/jvi.71.10.7567-7578.1997
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发表时间：
1997-10-01
影响因子：
5.4
通讯作者：
Masters, PS
中科院分区：
文献类型：
--
作者：
Hsue, B;Masters, PS
冠状病毒小鼠肝炎病毒（MHV）正义RNA基因组的3'非翻译区（UTR）包含合成负链病毒RNA所必需的序列以及可能对基因组和亚基因组正链RNA合成都至关重要的序列。我们发现MHV的整个3' UTR可以被牛冠状病毒（bovine coronavirus，BCV）的3' UTR所取代，其核苷酸序列总体上偏离了31%。两种被物种屏障隔开的病毒之间的这种交换是通过靶向RNA重组进行的。我们的结果定义了两个3'UTR的区域，尽管彼此存在大量序列取代、缺失或插入，但这些区域在功能上是等效的。更重要的是，我们尝试用BCV3 ' UTR的特定部分取代MHV 3' UTR的相应区域，导致发现了与核衣壳基因的终止密码子相邻的凸出茎环RNA二级结构，这是MHV病毒RNA复制所必需的。
The 3' untranslated region (UTR) of the positive-sense RNA genome of the coronavirus mouse hepatitis virus (MHV) contains sequences that are necessary for the synthesis of negative-strand viral RNA as well as sequences that may be crucial for both genomic and subgenomic positive-strand RNA synthesis. We have found that the entire 3' UTR of MHV could be replaced by the 3' UTR of bovine coronavirus (BCV), which diverges overall by 31% in nucleotide sequence. This exchange between two viruses that are separated by a species barrier was carried out by targeted RNA recombination. Our results define regions of the two 3' UTRs that are functionally equivalent despite having substantial sequence substitutions, deletions, or insertions with respect to each other. More significantly, our attempts to generate an unallowed substitution of a particular portion of the BCV 3' UTR for the corresponding region of the MHV 3' UTR led to the discovery of a bulged stem-loop RNA secondary structure, adjacent to the stop codon of the nucleocapsid gene, that is essential for MHV viral RNA replication.