Cloning and characterization of the mouse Fgf-1 gene.
Cloning and characterization of the mouse Fgf-1 gene.
复制标题
小鼠 Fgf-1 基因的克隆和表征。
DOI：
10.1016/s0378-1119(96)00365-4
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发表时间：
1996
期刊：
影响因子：
3.5
通讯作者：
Chiu,IM
中科院分区：
文献类型：
--
作者：
Madiai,F;Hackshaw,KV;Chiu,IM
成纤维细胞生长因子1（FGF-1或aFGF）是多种中胚层和神经外胚层来源的细胞的有丝分裂原，以及体内血管生成因子。它与血管生成性疾病包括动脉粥样硬化、癌症和炎性自身免疫性疾病有关。作为了解FGF-1在炎症病理生物学中作用的努力的一部分，我们分离并鉴定了小鼠FGF-1基因。使用小鼠FGF-1 cDNA作为探针的小鼠基因组DNA的Southern印迹分析显示，小鼠FGF-1由单拷贝基因编码。比较现有的小鼠Fgf-1 cDNA序列与新获得的基因组序列，使我们能够建立外显子/内含子边界。小鼠Fgf-1编码区由三个蛋白质编码外显子组成，我们确定它们被11.4-kb和4.9-kb的内含子分开。小鼠Fgf-1编码区的阐明揭示了小鼠和人Fgf-1基因结构之间的高度相似性。
Fibroblast growth factor 1 (FGF-1 or aFGF), is a mitogen for a variety of mesoderm- and neuroectoderm-derived cells, as well as an angiogenic factor in vivo. It has been implicated in angiogenic diseases including atherosclerosis, cancer and inflammatory autoimmune diseases. As part of an effort to understand the role of FGF-1 in the pathobiology of inflammation, we have isolated and characterized the mouse Fgf-1 gene. Southern blot analysis of mouse genomic DNA using the mouse Fgf-1 cDNA as a probe revealed that mouse FGF-1 is encoded by a single copy gene. Comparison of the available mouse Fgf-1 cDNA sequence with newly obtained genomic sequence allowed us to establish the exon/intron boundaries. The mouse Fgf-1 coding region is comprised of three protein coding exons, which we determined to be separated by an 11.4-kb and a 4.9-kb intron. The elucidation of the mouse Fgf-1 coding region revealed great similarity between the mouse and human Fgf-1 gene structure.