HPLC method for the analysis of harmol, harmalol, harmine and harmaline in the seeds of Peganum harmala L.
HPLC method for the analysis of harmol, harmalol, harmine and harmaline in the seeds of Peganum harmala L.
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DOI：
10.1016/s0731-7085(02)00568-x
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发表时间：
2003-02-26
影响因子：
3.4
通讯作者：
Kurucu, S
中科院分区：
文献类型：
--
作者：
Kartal, M;Altun, ML;Kurucu, S
建立了一种简便、灵敏的分离测定骆驼蓬碱、骆驼蓬碱、骆驼蓬碱和骆驼蓬碱的方法。采用Metasil ODS色谱柱，等度洗脱，流速1.5ml/min，分离骆驼蓬碱、骆驼蓬碱。流动相组成为异丙醇-乙腈-水-甲酸(100：100：300：0.3)(v/v)，用三乙胺调节pH至8.6。检测波长为330 nm。骆驼蓬碱、骆驼蓬碱的线性范围分别为9.375~250、30.750~246、31.250~500、31.000~248mug/ml。本方法适用于骆驼蓬种子中三尖杉酯酚、三尖杉酯酚、三尖杉酯碱和骆驼蓬碱的测定。
A simple and sensitive method for separation and determination of harmol, harmalol, harmine and harmaline has been developed and validated. Harmol, harmalol, harmine and harmaline were separated using a Metasil ODS column by isocratic elution with flow rate 1.5 ml/min. The mobile phase composition was Isopropyl alcohol-Acetonitrile-Water-Formic acid (100:100:300:0.3) (v/v/v/v) and pH adjusted 8.6 with triethylamine. Spectrophotometric detection was carried out at 330 nm. The linear range of detection for harmol, harmalol, harmine and harmaline were between 9.375-250, 30.750-246, 31.250-500 and 31.000-248 mug/ml, respectively. The method described was suitable for the determination of harmol, harmalol, harmine and harmaline in the seeds of Peganum harmala L. (C) 2002 Elsevier Science B.V. All rights reserved.