PLASMA-CLEARANCE KINETICS OF THE AMYLOID-RELATED HIGH-DENSITY LIPOPROTEIN APOPROTEIN, SERUM AMYLOID PROTEIN (APOSAA), IN THE MOUSE - EVIDENCE FOR RAPID APOSAA CLEARANCE
PLASMA-CLEARANCE KINETICS OF THE AMYLOID-RELATED HIGH-DENSITY LIPOPROTEIN APOPROTEIN, SERUM AMYLOID PROTEIN (APOSAA), IN THE MOUSE - EVIDENCE FOR RAPID APOSAA CLEARANCE
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DOI：
10.1172/jci110847
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发表时间：
1983-01-01
影响因子：
15.9
通讯作者：
BENDITT, EP
中科院分区：
文献类型：
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作者：
HOFFMAN, JS;BENDITT, EP
通过两种不同的方法在 BALB/c 小鼠中检查淀粉样蛋白相关的高密度脂蛋白 (HDL) 脱辅基蛋白血清淀粉样蛋白 (apoSAA) 的血浆清除动力学，使用标记的富含 125I-apoSAA 的 HDL 和未标记的血浆 apoSAA（通过放射免疫测定法监测清除率）。通过两种方法估计，apoSAA 的血浆半衰期约为 75-80 分钟，与 .apprx 的值相比。小鼠 apoA-I 11 小时。在痕量标记研究中，观察到两种主要 125I 标记的 apoSAA 同型的快速血浆清除率；这种代谢行为是 HDL 脱辅基蛋白中这些多肽所独有的。在用 HDL 纯化和重建 125I-apoSAA 时，快速血浆清除的特性消失，表明该特性对变性条件不稳定。旨在确定 125I-apoSAA 代谢命运的研究没有提供与其他 125I-HDL 脱辅基蛋白相比 125I-apoSAA 选择性排泄或清除到独特组织部位的证据。
The plasma clearance kinetics of the amyloid-related high density lipoprotein (HDL) apoprotein serum amyloid protein (apoSAA) was examined in BALB/c mice by 2 different methods, using labeled 125I-apoSAA-rich HDL and unlabeled plasma apoSAA (clearance monitored by radioimmunoassay). The plasma half-life of apoSAA, estimated by both methods, was on the order of 75-80 min, as compared with a value of .apprx. 11 h for mouse apoA-I. In trace-labeling studies, the rapid plasma clearance of both major 125I-labeled apoSAA isotypes was observed; this metabolic behavior was unique to these polypeptides among HDL apoproteins. The property of rapid plasma clearance was lost upon purification and reconstitution of 125I-apoSAA with HDL, indicating that this property is labile to denaturing conditions. Studies aimed at determining the metabolic fate of 125I-apoSAA gave no evidence for either the selective excretion of 125I-apoSAA or clearance to unique tissue sites as compared with other 125I-HDL apoproteins.