BRDU-HOECHST FLOW-CYTOMETRY - A UNIQUE TOOL FOR QUANTITATIVE CELL-CYCLE ANALYSIS
BRDU-HOECHST FLOW-CYTOMETRY - A UNIQUE TOOL FOR QUANTITATIVE CELL-CYCLE ANALYSIS
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DOI：
10.1016/0014-4827(88)90302-3
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发表时间：
1988-02-01
影响因子：
3.7
通讯作者：
HOEHN, H
中科院分区：
文献类型：
--
作者：
RABINOVITCH, PS;KUBBIES, M;HOEHN, H
与其他技术不同的是，brdu猝灭33258 Hoechst荧光的流式细胞术分析可用于测量人外周血淋巴细胞生长刺激后连续三个细胞周期中的细胞活化和G1、S和G2/M室分布。细胞周期动力学曲线可以从BrdU-Hoechst流动数据构建，可以同时评估生长分数、滞后时间、室存在率、室持续时间和室停。这种新的多功能技术的应用包括药物和生长因子效应的评估，细胞老化，以及医学和免疫学的诊断。
Unlike other techniques, flow cytometric analysis of BrdU-quenched 33258 Hoechst fluorescence may be used to measure cell activation and the G1, S, and G2/M compartment distributions in each of three successive cell cycles after growth stimulation of human peripheral blood lymphocytes. Cell cycle kinetic curves can be constructed from the BrdU-Hoechst flow data which allow the simultaneous assessment of growth fraction, lagtime, compartment exist rate, compartment duration, and compartment arrest. Applications of this new versatile technique include the evaluation of drug and growth factor effects, cell aging, and diagnosis in medicine and immunology.