Physiological and Molecular Dissection of Daily Variance in Exercise Capacity
Physiological and Molecular Dissection of Daily Variance in Exercise Capacity
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DOI：
10.1016/j.cmet.2019.03.012
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发表时间：
2019-07-02
期刊：
影响因子：
29
通讯作者：
Asher, Gad
中科院分区：
文献类型：
--
作者：
Ezagouri, Saar;Zwighaft, Ziv;Asher, Gad
身体表现依赖于无数反应的协同作用，其中许多反应是在生物钟控制下的。然而，在分子水平上对运动表现的时间依赖性影响知之甚少。我们发现，小鼠和人类在活动期的早期和后期表现出白天运动能力的差异。小鼠白天的变化取决于运动强度和生物钟蛋白PER1/2。骨骼肌的高通量基因表达和代谢谱揭示了在白天依赖的方式下运动时不同的代谢途径。值得注意的是，我们发现ZMP是一种内源性AMPK激活剂，通过运动以时间依赖的方式诱导，调节糖酵解和脂肪酸氧化途径的关键步骤，并可能增强运动能力。总的来说，我们认为一天中的时间是运动能力和相关代谢途径的主要调节因素。
Physical performance relies on the concerted action of myriad responses, many of which are under circadian clock control. Little is known, however, regarding the time-dependent effect on exercise performance at the molecular level. We found that both mice and humans exhibit daytime variance in exercise capacity between the early and late part of their active phase. The daytime variance in mice was dependent on exercise intensity and relied on the circadian clock proteins PER1/2. High-throughput gene expression and metabolic profiling of skeletal muscle revealed metabolic pathways that are differently activated upon exercise in a daytime-dependent manner. Remarkably, we discovered that ZMP, an endogenous AMPK activator, is induced by exercise in a time-dependent manner to regulate key steps in glycolytic and fatty acid oxidation pathways and potentially enhance exercise capacity. Overall, we propose that time of day is a major modifier of exercise capacity and associated metabolic pathways.