Overexpression of HOXB5, Cyclin D1 and PCNA in Congenital Cystic Adenomatoid Malformation
Overexpression of HOXB5, Cyclin D1 and PCNA in Congenital Cystic Adenomatoid Malformation
复制标题
DOI：
10.1159/000322738
复制
发表时间：
2011-01-01
影响因子：
2.2
通讯作者：
Baxi, Laxmi V.
中科院分区：
文献类型：
--
作者：
Wang, Xiangyuan;Wolgemuth, Debra J.;Baxi, Laxmi V.
目的：研究HOXB5、cyclin D1和增殖细胞核抗原（PCNA）蛋白在人先天性囊性腺瘤样畸形（CCAM）中的表达规律，为其病因的分子基础奠定基础。方法：采用HOXB5、cyclin D1和PCNA蛋白抗体对CCAM冰冻标本和正常肺组织进行免疫组化处理。结果：免疫组化显示，与正常相邻对照肺组织相比，CCAM组织间充质细胞中HOXB5、cyclin D1和PCNA的表达水平明显升高。结论：肺组织细胞HOXB5、cyclin D1、PCNA免疫组化检测水平升高是CCAM肺组织细胞的特征。这种升高的HOXB5表达可能与CCAM疾病中观察到的异常细胞分化有关。cyclin D1和PCNA的表达升高进一步表明细胞增殖的增加有助于CCAM肺组织的过度生长。巴塞尔S. Karger股份有限公司版权所有
Objective: We investigated the patterns of expression of HOXB5, cyclin D1 and proliferating cell nuclear antigen (PCNA) proteins in human congenital cystic adenomatoid malformation (CCAM) to establish the molecular basis of its etiology. Methods: Immunohistochemistry was performed on frozen archival specimens of CCAM and normal lung tissue as controls using antibodies against HOXB5, cyclin D1 and PCNA proteins. Results: Immunohistochemistry revealed a higher level of expression of HOXB5, cyclin D1 and PCNA predominantly in mesenchymal cells of the CCAM tissues as compared to normal adjacent control lung tissues. Conclusion: Elevated levels of immunohistochemical detection of HOXB5, cyclin D1 and PCNA were characteristic properties of lung tissue cells in CCAM. This elevated HOXB5 expression may correlate with the aberrant cellular differentiation observed in the CCAM disorder. Elevated expression of cyclin D1 and PCNA further suggests that increased cellular proliferation contributes to the overgrowth of lung tissue in CCAM. Copyright (C) 2011 S. Karger AG, Basel