Interactions between the ovary and the local IGF-I axis modulate mammary development in prepubertal heifers
Interactions between the ovary and the local IGF-I axis modulate mammary development in prepubertal heifers
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DOI：
10.1677/joe.0.1770295
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发表时间：
2003-05-01
影响因子：
4
通讯作者：
Akers, RM
中科院分区：
文献类型：
--
作者：
Berry, SDK;Howard, RD;Akers, RM
目的是确定卵巢切除术和上皮-基质相互作用对青春期前小母牛乳腺发育和IGF-I和IGFBP mRNA局部表达的影响。在14头1-3月龄荷斯坦小母牛的两个腺体中制备无上皮（“清除”）脂肪垫（CFP）。其中8头小牛还进行了卵巢切除术。血清GH，IGF-I和催乳素浓度不受卵巢切除术的影响。在6月龄时，处死小牛以提供实质、CFP和完整脂肪垫（MFP）的乳房样品。切除卵巢的小牛乳房总质量减少（130 +/- 21 g vs 304 +/- 25 g; P
The objective was to determine the effects of ovariectomy and epithelial-stromal interactions on mammary development and local expression of IGF-I and IGF-binding protein (IGFBP) mRNA in prepubertal heifers. An epithelium-free ('cleared') fat pad (CFP) was prepared in two glands in each of 14 Holstein heifers, aged 1-3 months. Eight of the calves were also ovariectomized. Serum concentrations of GH, IGF-I and prolactin were not affected by ovariectomy. At 6 months of age, calves were killed to provide mammary samples of parenchyma, CFP and intact fat pad (MFP). Total mammary mass was reduced in ovariectomized calves (130 +/- 21 g vs 304 +/- 25 g; P