Tea catechins prevent the development of atherosclerosis in apoprotein E-deficient mice
Tea catechins prevent the development of atherosclerosis in apoprotein E-deficient mice
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DOI：
10.1093/jn/131.1.27
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发表时间：
2001-01-01
影响因子：
4.2
通讯作者：
Tomita, T
中科院分区：
文献类型：
--
作者：
Miura, Y;Chiba, T;Tomita, T
绿色茶含有各种抗氧化黄烷-3醇（茶儿茶素），如（-）-表没食子儿茶素没食子酸酯（EGCg，主要的儿茶素），其在体外和离体人体中对LDL氧化产生有效的抑制作用。在这项研究中，茶儿茶素的抗动脉粥样硬化作用进行了检查，在动脉粥样硬化易感的C57 BL/6 J，载脂蛋白（apo）E-缺陷小鼠。雄性apoE缺陷小鼠（10周龄）喂食致动脉粥样硬化饮食14周;在此期间，一组（茶）提供补充有绿色茶提取物（0.8 g/L）的饮用水，另一组（对照）仅提供载体。茶提取物由以下组成（g/100 g）：EGCg，58.4;（-）-表没食子儿茶素（EGC），11.7;（-）-表儿茶素（EC），6.6;（-）-没食子儿茶素没食子酸酯（GCg），1.6;（-）-表儿茶素没食子酸酯（ECg），0.5;和咖啡因，0.4。实际摄入儿茶素的估计值为1.7mg/d。小鼠）。茶的摄入并不影响血浆胆固醇或甘油三酯浓度。在第8周，茶组的血浆过氧化脂质减少，表明茶摄入改善了体内氧化状态。与对照组相比，茶组从主动脉弓到股动脉分叉的动脉粥样硬化面积和主动脉重量均显著减轻23%。主动脉胆固醇和甘油三酯含量分别降低27%和50%，在茶组比对照组。这些结果表明，长期摄入茶提取物可以防止动脉粥样硬化的发展，而不改变载脂蛋白E缺陷小鼠的血脂水平，可能是通过茶的有效抗氧化活性。
Green tea contains various antioxidative flavan-3ols (tea catechins), such as (-)-epigallocatechin gallate (EGCg, the major catechin), which exert potent inhibitory effects on LDL oxidation in vitro and ex vivo in humans. In this study, the antiatherogenic effects of tea catechins were examined in atherosclerosis-susceptible C57BL/6J, apoprotein (apo)E-deficient mice. Male apoE-deficient mice (10 wk old) were fed an atherogenic diet for 14 wk; during that time, one group (tea) was supplied drinking water supplemented with green tea extract (0.8 g/L), and another group (control) was offered the vehicle only. The tea extract consisted of the following (g/100 g): EGCg, 58.4; (-)-epigallocatechin (EGC), 11.7; (-)-epicatechin (EC), 6.6; (-)-gallocatechingallate (GCg), 1.6; (-)-epicatechin gallate (ECg), 0.5; and caffeine, 0.4. The estimated actual intake of tea catechin was 1.7 mg/(d . mouse). Tea ingestion did not influence plasma cholesterol or triglyceride concentrations. Plasma lipid peroxides were reduced in the tea group at wk 8, suggesting that the in vivo oxidative state is improved by tea ingestion. Atheromatous areas in the aorta from the arch to the femoral bifurcation and aortic weights were both significantly attenuated by 23% in the tea group compared with the control group. Aortic cholesterol and triglyceride contents were 27 and 50% lower, respectively, in the tea group than in the control group. These results suggest that chronic ingestion of tea extract prevents the development of atherosclerosis without changing the plasma lipid level in apoE-deficient mice, probably through the potent antioxidative activity of the tea.