Peanut agglutinin, a new mitogen that binds to galactosyl sites exposed after neuraminidase treatment.
Peanut agglutinin, a new mitogen that binds to galactosyl sites exposed after neuraminidase treatment.
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DOI：
10.4049/jimmunol.115.5.1243
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发表时间：
1975-11
影响因子：
4.4
通讯作者：
A. Novogrodsky;R. Lotan;A. Ravid;N. Sharon
中科院分区：
文献类型：
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作者：
A. Novogrodsky;R. Lotan;A. Ravid;N. Sharon
花生凝集素经亲和层析纯化后，仅在用神经氨酸酶处理后才能凝集小鼠、大鼠、豚鼠和人的淋巴细胞。然而，它只刺激神经氨酸酶处理的大鼠和人类细胞。在神经氨酸酶处理的大鼠和小鼠淋巴细胞上发现了类似数量的花生凝集素细胞表面受体，尽管后者的细胞没有受到凝集素的刺激。半乳糖特异性抑制花生凝集素对淋巴细胞的凝集和刺激作用。用神经氨酸酶和β-半乳糖苷酶顺序处理淋巴细胞显著降低了细胞对花生凝集素、大豆凝集素和半乳糖氧化酶刺激的反应。这表明，相同的半乳糖残基可能是上述有丝分裂原触发淋巴细胞的初始步骤的目标。
Peanut agglutinin, purified by affinity chromatography, agglutinates lymphocytes from mouse, rat, guinea pig, and man only after their treatment with neuraminidase. However, it stimulates only neuraminidase-treated rat and human cells. A similar number cell surface receptors for peanut agglutinin was found on neuraminidase-treated rat and mouse lymphocytes although the latter cells were not stimulated by the lectin. Galactose specifically inhibited the agglutination and stimulation of lymphocytes by peanut agglutinin. Sequential treatment of lymphocytes with neuraminidase and beta-galactosidase markedly reduced the response of the cells to stimulation by peanut agglutinin, soybean agglutinin, and galactose oxidase. It is suggested that the same galactosyl residue may be the target for the initial step in triggering lymphocytes by the above mentioned mitogens.