In vitro mechanical and biological assessment of hydroxyapatite-reinforced polyethylene composite
In vitro mechanical and biological assessment of hydroxyapatite-reinforced polyethylene composite
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DOI：
10.1023/a:1018516813604
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发表时间：
1997-12-01
影响因子：
3.7
通讯作者：
Bonfield, W
中科院分区：
文献类型：
--
作者：
Huang, J;DiSilvio, L;Bonfield, W
从力学和生物学两方面对羟基磷灰石（HA）增强聚乙烯（PE）复合材料（HAPEX（TM））的体外性能进行了表征。HAPEX（TM）的机械性能，例如拉伸强度和杨氏模量，在37和70摄氏度的生理溶液中浸泡不同时间后几乎没有变化。此外，通过测量DNA合成和成骨细胞表型表达来评估体外原代人成骨细胞样（HOB）细胞对HAPEX（TM）的生物学反应。HAPEX（TM）上的细胞增殖率通过DNA含量随时间的增加来证明。在第1天观察到HAPEX（TM）的高氚化胸苷（[H-3]-TdR）掺入/DNA速率，表明对细胞增殖的刺激作用。碱性磷酸酶（ALP）活性在HAPEX（TM）上比在未填充的PE上更早地表达，并且随时间增加，表明HOB细胞已经开始分化。此外，发现复合材料中的HA颗粒为细胞附着提供了有利的位点。似乎HA颗粒在HAPEX（TM）中的存在可能具有作为体内骨结合的微锚的优点。
In vitro performance of hydroxyapatite (HA)-reinforced polyethylene (PE) composite (HAPEX(TM)) has been characterized from both mechanical and biological aspects. The mechanical properties of HAPEX(TM), such as tensile strength and Young's modulus, showed little change after immersion in a physiological solution at 37 and 70 degrees C for various periods. In addition, the biological response of primary human osteoblast-like (HOB) cells in vitro on HAPEX(TM) was assessed by measuring DNA synthesis and osteoblast phenotype expression. Cell proliferation rate on HAPEX(TM) was demonstrated by an increase in DNA content with time. A high tritiated thymidine ([H-3]-TdR) incorporation/DNA rate was observed on day 1 for HAPEX((TM)), indicating a stimulatory effect on cell proliferation. The alkaline phosphatase (ALP) activity was expressed earlier on HAPEX(TM) than on unfilled PE and increased with time, indicating that HOB cells had commenced differentiation. Furthermore, it was found that the HA particles in the composite provided favourable sites for cell attachment. It appears that the presence of HA particles in HAPEX(TM) may have the advantage of acting as microanchors for bone bonding in vivo.