Evaluation of autologous bone marrow in wound healing in animal model: a possible application of autologous stem cells
Evaluation of autologous bone marrow in wound healing in animal model: a possible application of autologous stem cells
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DOI：
10.1111/j.1742-481x.2011.00909.x
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发表时间：
2012-10-01
影响因子：
3.1
通讯作者：
Pradhan, Nisith Ranjan
中科院分区：
文献类型：
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作者：
Akela, Ashok;Nandi, Samit Kumar;Pradhan, Nisith Ranjan
进行了一项研究，以评估自体骨髓来源细胞与自体血液血沉棕黄层相比在兔模型中快速皮肤伤口愈合的潜力。选取15只实验动物（兔子），随机分为三组，制作三个方形全层皮肤切除伤口。用血浆中的自体骨髓细胞（组1）、血浆中的血沉棕黄层（组2）和作为对照的自体血浆（组3）治疗伤口。观察伤口30天，进行肉芽组织形成、生化、组织形态学和组织化学评估。在这项研究中，第 3 组动物伤口中的肉芽组织明显较少，其次是第 2 组和第 1 组动物。与其他组相比，第1组的新血管形成、肉芽组织形成、更致密、更粗和排列更好的胶原纤维、网状纤维和弹性蛋白纤维形成更多。结论是，与自体血液和自体血浆的血沉棕黄层（对照）相比，将骨髓来源的有核细胞应用到伤口边缘可导致早期且明显更快的完全愈合速度。这种方法可能对愈合速度较慢的各种表面伤口有益，并推荐用于将来治疗各种复杂伤口。
A study was conducted to evaluate the potential of autologous bone marrow-derived cells in comparison with buffy coat of autologous blood for rapid cutaneous wound healing in rabbit model. Three square full-thickness skin excisional wounds were created in 15 selected experimental animals (rabbit) divided randomly into three groups. The wound was treated with autologous bone marrow cells in plasma (group 1), buffy coat of blood in plasma (group 2) and autologous plasma as control (group 3). Wounds were observed for 30 days for granulation tissue formation, biochemical, histomorphological and histochemical evaluation. In this study, granulation tissue appeared significantly lesser in wounds of group 3 animals followed by group 2 and 1 animals. Neovascularisation, granulation tissue formation, denser, thicker and better arranged collagen fibres, reticulin fibres and elastin fibres formation was more in group 1 as compared with other groups. It was concluded that the application of bone marrow-derived nucleated cells into the wound margins resulted in early and significantly faster rate of complete healing as compared with buffy coat of autologous blood and autologous plasma (control). This approach may be beneficial in various surface wounds that heal at a slower rate and recommended for healing of various complicated wound in future.