Expression and network analysis of genes related to melanocyte development in the Silky Fowl and White Leghorn embryos
Expression and network analysis of genes related to melanocyte development in the Silky Fowl and White Leghorn embryos
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DOI：
10.1007/s11033-010-0248-2
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发表时间：
2011-02-01
影响因子：
2.8
通讯作者：
Deng, Xuemei
中科院分区：
文献类型：
--
作者：
Li, Yulin;Zhu, Xuping;Deng, Xuemei
丝鸡是一种天然突变体，具有多种内部组织色素沉着。虽然色素沉着的机制尚不清楚，但最近的研究表明，成黑素细胞的异常迁移和环境屏障分子的缺乏是导致丝鸡色素沉着的原因。本研究选取13个与黑素细胞发育相关的基因，包括SRY-box 10 （Sox10）、配对盒（Pax3）、干细胞因子（Scf）、v-kit Hardy-Zuckerman 4猫肉瘤病毒癌基因同源物（Kit）、内皮素b型受体（Ednrb）、内皮素3 （Edn3）、小眼相关转录因子（Mitf）、酪氨酸酶（Tyr）、酪氨酸酶相关蛋白-1 （Trp1）、酪氨酸酶相关蛋白-2 （Trp2）、黑素皮质素-1受体（Mc1r）、agouti相关蛋白（Agrp）和原黑素皮质素（Pomc）]。在孵育2.5 ~ 15天的11个阶段检测到转录物的表达。在这些胚胎时期，Mitf、Kit、Scf和Agrp在白来窝鸡中比在白来窝鸡中表达得更早。与黑素细胞增殖和分化有关的Sox10、Ednrb、Kit、Mc1r和Agrp在孵育5 ~ 6 d时在乌鸡中的表达高于白来窝（P < 0.05）。孵育第8 d， Mitf、Tyr、Trp1、Trp2和Mc1r在白来窝鸡中的表达量高于白来窝鸡（P < 0.05）， Agrp在白来窝鸡中的表达量高于白来窝鸡（P < 0.05）。此外，基于表达数据构建了黑素细胞发育的调控网络。该网络预测了新的调节关系，并证实了已报道的关系。这些结果为乌鸡色素沉着的分子机制提供了生物学视角。然而，需要进一步的调查来证实这些调节关系。
Silky Fowl is a natural mutant with hyperpigmentation of various internal tissues. Although the mechanism of hyperpigmentation remains unclear, recent studies have shown that the abnormal migration of melanoblast and the absence of environmental barrier molecules are responsible for the hyperpigmentation in Silky Fowl. In this study, 13 genes related to melanocyte development were selected to detect expression changes between Silky Fowl and White Leghorn [including SRY-box 10 (Sox10), paired box (Pax3), stem cell factor (Scf), v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog (Kit), endothelin type-B receptor (Ednrb), endothelin 3 (Edn3), microphthalmia-associated transcription factor (Mitf), tyrosinase (Tyr), tyrosinase-related protein-1 (Trp1), tyrosinase-related protein-2 (Trp2), melanocortin-1 receptor (Mc1r), Agouti-related proteins (Agrp), and Proopiomelanocortin (Pomc)]. Transcript expression was detected in 11 stages from 2.5 to 15 days of incubation. In these embryonic periods, Mitf, Kit, Scf, and Agrp expressed earlier in Silky Fowl than in White Leghorn. Sox10, Ednrb, Kit, Mc1r, and Agrp, associating with the proliferation and differentiation of melanoblast, expressed higher (P < 0.05) in Silky Fowl than White Leghorn during 5-6 days of incubation. After day 8 of incubation, Mitf, Tyr, Trp1, Trp2, and Mc1r expressed higher (P < 0.05) in Silky Fowl than White Leghorn, while Agrp expressed higher (P < 0.05) in White Leghorn than Silky Fowl. Moreover, a regulatory network for melanocyte development was constructed based on the expression data. The network predicted novel regulatory relationships and confirmed relationships that have been reported. These results provide biological insight into the molecular mechanism of hyperpigmentation in the Silky Fowl. However, further investigation is needed to confirm these regulatory relationships.