Tissue-specific developmental expression of OAX, a Xenopus repetitive element.
Tissue-specific developmental expression of OAX, a Xenopus repetitive element.
复制标题
OAX（非洲爪蟾重复元件）的组织特异性发育表达。
DOI：
10.1016/s0925-4773(00)00307-5
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发表时间：
2000
影响因子：
2.6
通讯作者：
Saha,MS
中科院分区：
文献类型：
--
作者：
Whitford,KL;Oakes,JA;Scholnick,J;Saha,MS
非洲爪蟾基因组的大约1%由高度重复的DNA组成，这些DNA被称为OAX（非洲爪蟾卵母细胞激活）、卫星I或重复HindIII MST 2。OAX以大约750个碱基对的串联重复单位存在，编码由RNA聚合酶III转录的小RNA种类家族。尽管许多关于早期胚胎基因调控的经典研究的主题，但关于OAX表达的报道仍然是矛盾和不完整的。因此，我们使用整装原位杂交和RNA酶保护试验，详细研究了OAX在非洲爪蟾胚胎的各个阶段的表达模式。OAX最初在原肠胚阶段表达;到尾芽阶段，胚胎在骨水泥腺的背侧边界、发育中的体节和分化中的骨骼肌以及神经管的背侧显示离散的表达区。这些数据表明，OAX在严格的空间和时间调控下以动态模式表达。
Approximately 1% of the Xenopus laevis genome consists of highly repetitive DNA known alternatively as OAX (for Oocyte Activation in Xenopus), Satellite I, or Repetitive HindIII Monomer 2. Present as tandemly repeated units of approximately 750 base pairs, OAX encodes a family of small RNA species transcribed by RNA polymerase III. Although the subject of many of the classic studies on early embryonic gene regulation, reports on OAX expression remain contradictory and incomplete. Using whole-mount in situ hybridization and RNase protection assays, we have therefore examined in detail the expression pattern of OAX in Xenopus embryos of various stages. OAX is initially expressed during gastrula stages; by tailbud stages embryos display discrete zones of expression at the dorsal boundary of the cement gland, in the developing somites and differentiating skeletal muscle, as well as in the dorsal aspect of the neural tube. These data demonstrate that OAX is expressed in a dynamic pattern under tight spatial and temporal regulation.