Effect of the semen extract of Cuscuta chinensis on inflammatory responses in LPS-stimulated BV-2 microglia
Effect of the semen extract of Cuscuta chinensis on inflammatory responses in LPS-stimulated BV-2 microglia
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DOI：
10.1016/s1875-5364(14)60088-1
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发表时间：
2014-08-01
影响因子：
4.6
通讯作者：
Park, Yong-Ki
中科院分区：
文献类型：
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作者：
Kang, Seok Yong;Jung, Hyo Won;Park, Yong-Ki
目的：研究菟丝子精液提取物的抗炎作用。（Cuscutae Semen； CS）对脂多糖（LPS）刺激的BV-2小胶质细胞产生炎症介质、一氧化氮（NO）、前列腺素2 （PGE(2)）和促炎细胞因子的影响。方法：用CS提取物处理BV-2细胞30 min，然后用LPS或不加LPS刺激BV-2细胞24 h，采用Griess法和ELISA法检测NO、PGE(2)和促炎因子水平。采用RT-PCR和Western blot分别检测诱导型一氧化氮合酶（iNOS）和环氧化酶(COX)-2 mRNA和蛋白的表达。Western blot检测细胞外信号调节激酶1/2 （ERK1/2）、Jun n-末端激酶（JNK）和p38丝裂原活化蛋白激酶（MAPK）的磷酸化水平以及核因子(NF)- κ B p65的核表达水平。结果：CS提取物通过抑制活化小胶质细胞iNOS和COX-2的表达，显著降低NO和PGE(2)的生成。CS提取物通过下调tnf - α、IL-1 β和IL-6的转录水平，降低了它们的产生。此外，CS提取物还抑制了活化小胶质细胞中ERK1/2、JNK和p38 MAPK的磷酸化，以及NF-kappa B p65的核易位。结论：这些结果表明，CS提取物能够通过激活小胶质细胞来抑制炎症反应，提示CS提取物具有治疗脑炎症的潜力
AIM: To investigate the anti-inflammatory activities of the semen extract of Cuscuta chinensis Lam. (Cuscutae Semen; CS) on the production of inflammatory mediators, nitric oxide (NO), prostaglandin 2 (PGE(2)), and proinflammatory cytokines in lipopolysaccharide (LPS)-stimulated BV-2 microglia.METHOD: BV-2 cells were treated with CS extract for 30 min, and then stimulated with LPS or without for 24 h. The levels of NO, PGE(2) and proinflammatory cytokines were measured by Griess assay and ELISA. The expression of inducible nitric oxide synthase (iNOS), and cyclooxygenase (COX)-2 mRNA and protein was determined by RT-PCR and Western blot, respectively. The phosphorylation of extracellular signal-regulated kinase 1/2 (ERK1/2), Jun N-terminal kinase (JNK), and p38 mitogen-activated protein kinase (MAPK), and the nuclear expression of nuclear factor (NF)-kappa B p65 were investigated by Western blot analysis.RESULTS: CS extract significantly decreased the production of NO and PGE(2) by suppressing the expression of iNOS and COX-2 in activated microglia. CS extract decreased the production of TNF-alpha, IL-1 beta, and IL-6 by down-regulating their transcription levels-In addition, CS extract suppressed the phosphorylation of ERK1/2, JNK, and p38 MAPK, and the nuclear translocation of NF-kappa B p65 in activated microglia.CONCLUSION: These results indicate that CS extract is capable of suppressing the inflammatory response by microglia activation, suggesting that CS extract has potential in the treatment of brain inflammation