Emergence of epidemic O'nyong-nyong fever in Uganda after a 35-year absence: Genetic characterization of the virus
Emergence of epidemic O'nyong-nyong fever in Uganda after a 35-year absence: Genetic characterization of the virus
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DOI：
10.1006/viro.1998.9437
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发表时间：
1998-12-05
期刊：
影响因子：
3.7
通讯作者：
Miller, BR
中科院分区：
文献类型：
--
作者：
Lanciotti, RS;Ludwig, ML;Miller, BR
O‘nyong-Nyong(ONN)病毒是一种属于塞姆利基森林病毒(SFV)抗原复合体的甲型病毒。ONN最初于1959年在乌干达北部被分离出来，当时正值虫媒病毒暴发流行的早期阶段，报告了200万例。直到1996年从乌干达南部的一次流行病中分离出来，才报告有新的ONN流行或人类隔离病例。我们报道了其中一个1996-1997年的On分离株(SG650)和与之相关的甲型病毒Igbo Ora病毒的全核苷酸和推导的氨基酸序列。这些数据表明，最近的ONN病毒分离株与之前发表的1959年分离的ONN毒株密切相关。此外，对序列数据的系统发育分析表明，先前被认为是与ONN和基孔肯雅(Chik)密切相关的独立病毒的Igbo Ora病毒显然是On N的一个毒株。序列数据还显示，与已发表的On(1959)序列不同，1996-1997疫情的所有ONN毒株在nsp3-nsp4连接处都有一个终止密码子。(C)1998年学术出版社。
O'nyong-nyong (ONN) virus is an alphavirus (family Togaviridae, genus Alphavirus) classified in the Semliki Forest virus (SFV) antigenic complex. ONN was initially isolated in northern Uganda in 1959 during the early stages of an explosive arbovirus epidemic in which >2 million cases were reported. No additional epidemics or human isolations of ONN were reported until 1996, when it was isolated from an epidemic in southern Uganda. We report the complete nucleotide and deduced amino acid sequence of one of these 1996-1997 ONN isolates (SG650) and that of the related alphavirus Igbo Ora virus. The data indicate that the recent ONN virus isolate is closely related to the previously published ONN strain isolated in 1959. In addition, phylogenetic analysis of the sequence data reveals that Igbo Ora virus, previously thought to be a separate virus closely related to ONN and Chikungunya (CHIK), clearly is a strain of ON N. The sequence data also reveal that unlike the published ONN (1959) sequence, all ONN strains from the 1996-1997 epidemic possess a stop codon at the nsp3-nsp4 junction. (C) 1998 Academic Press.