Reconstitution of retinoid X receptor function and combinatorial regulation of other nuclear hormone receptors in the yeast Saccharomyces cerevisiae.
Reconstitution of retinoid X receptor function and combinatorial regulation of other nuclear hormone receptors in the yeast Saccharomyces cerevisiae.
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酿酒酵母中视黄醇 X 受体功能的重建和其他核激素受体的组合调节。
DOI：
10.1073/pnas.90.15.6929
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发表时间：
1993
影响因子：
11.1
通讯作者：
Privalsky,ML
中科院分区：
文献类型：
--
作者：
Hall,BL;Smit-McBride,Z;Privalsky,ML
核激素受体家族的转录因子通过一个复杂的相互作用组合网络调节基因表达。特别令人感兴趣的是类维甲酸X受体（RXRs）与维甲酸受体（RARs）和甲状腺激素受体（TRs）形成异源二聚体的能力，从而改变其活性。我们在此报道RXR， RAR和TR功能可以在酵母酵母中重建，并证明在脊椎动物细胞中看到的组合调节可以在酵母背景下复制。使用这个系统，我们已经证明RARs对多种类视黄酸配体有反应，但RXRs对视黄酸的9-顺式异构体有特异性。RXR增强了RARs和TRs对多种激素反应元件的活性，但没有明显改变它们的DNA特异性。有趣的是，RXR增强RARs和TRs激活基因的能力因不同的受体亚型而异。
The nuclear hormone receptor family of transcription factors regulates gene expression via a complex combinatorial network of interactions. Of particular interest is the ability of retinoid X receptors (RXRs) to form heterodimers with retinoic acid receptors (RARs) and thyroid hormone receptors (TRs), thereby modifying their activities. We report here that RXR, RAR, and TR function can be reconstituted in the yeast Saccharomyces cerevisiae and demonstrate that the combinatorial regulation seen in vertebrate cells can be reproduced in the yeast background. Using this system, we have shown that RARs respond to a wide variety of retinoid ligands but that RXRs are specific for the 9-cis isomer of retinoic acid. RXR enhanced the activity of RARs and TRs on a variety of hormone response elements without demonstrably altering their DNA specificity. Interestingly, the ability of RXR to potentiate gene activation by RARs and by TRs varied for different receptor isoforms.