Role of phenylalanine ammonia-lyase in heat pretreatment-induced chilling tolerance in banana fruit
Role of phenylalanine ammonia-lyase in heat pretreatment-induced chilling tolerance in banana fruit
复制标题
苯丙氨酸解氨酶在热预处理诱导的香蕉果实耐冷性中的作用。
DOI：
10.1111/j.1399-3054.2007.01013.x
复制
发表时间：
2008-03-01
影响因子：
6.4
通讯作者：
Lu, Wang-jin
中科院分区：
文献类型：
--
作者：
Chen, Jian-ye;He, Li-hong;Lu, Wang-jin
越来越多的证据表明，苯丙氨酸解氨酶（PAL，EC 4.3.1.5）与植物组织的低温胁迫有关。香蕉果实对冷害高度敏感。然而，对PAL在果实低温中的作用（即基因表达、蛋白水平和活性）知之甚少。在这项工作中，PAL的参与诱导的热处理（38 ℃，3天）之前，储存（8 ℃）的耐冷性进行了研究。同时，利用PAL抑制剂2-氨基茚满-2-膦酸（AIP）进一步研究了PAL在抗冷性中的作用。结果表明，低温贮藏过程中，苹果PAL mRNA转录本（MaPAL 1和MaPAL 2）和PAL蛋白表达量增加。贮藏前的热处理减轻了冷害，提高了PAL活性、蛋白质含量和MaPAL 1和MaPAL 2转录水平。AIP处理抑制了高温预处理后PAL参数的升高，加重了冷害。因此，这些研究结果表明，PAL的诱导热预处理在转录和翻译水平上的调节，PAL可能在热预处理诱导香蕉果实的耐冷性中发挥作用。
Increasing evidence suggests that phenylalanine ammonia-lyase (PAL, EC 4.3.1.5) is associated with low temperature stress in plant tissues. Banana fruit are highly susceptible to chilling injury. However, little is known about the role of PAL (i.e. gene expression, protein level and activity) in fruit chilling. In this work, the involvement of PAL induced by heat treatment (38 degrees C for 3 days) prior to storage (8 degrees C) in chilling tolerance was investigated. The PAL inhibitor 2-aminoindan-2-phosphonic acid (AIP) was also used to further study the role of PAL in the chilling tolerance. The results showed that mRNA transcripts (MaPAL1 and MaPAL2) and PAL protein levels increased during storage at chilling temperature. Heat treatment prior to storage alleviated chilling injury and enhanced PAL activity, protein amount and MaPAL1 and MaPAL2 transcript levels. The increases in parameters of PAL upon heat pretreatment were all inhibited by AIP treatment, which resulted in aggravation of chilling injury. Thus, these findings indicate that the induction of PAL by heat pretreatment was regulated at both the transcriptional and the translational levels and that PAL may play a role in heat pretreatment-induced chilling tolerance of banana fruit.