CircRNA8924 Promotes Cervical Cancer Cell Proliferation, Migration and Invasion by Competitively Binding to MiR-518d-5p /519-5p Family and Modulating the Expression of CBX8
CircRNA8924 Promotes Cervical Cancer Cell Proliferation, Migration and Invasion by Competitively Binding to MiR-518d-5p /519-5p Family and Modulating the Expression of CBX8
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DOI：
10.1159/000491716
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发表时间：
2018-01-01
影响因子：
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通讯作者：
Li, Weishan
中科院分区：
文献类型：
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作者：
Liu, Jiamei;Wang, Danbo;Li, Weishan
背景/目标：环状 RNA (circRNA) 在各种人类癌症的发生和进展中发挥着重要作用。然而，circRNA在宫颈癌（CC）中的表达和功能却很少被探索。本研究的目的是研究circRNA8924在CC中的生物学功能并阐明所涉及的可能的分子机制。方法：采用定量聚合酶链反应测定CC组织和细胞中circRNA8924、miR-518d-5p/519-5p和CBX8的mRNA表达量。通过蛋白质印迹法测量CBX8蛋白表达。 CCK-8测定用于评估细胞增殖，transwell测定用于确定细胞迁移和侵袭。采用荧光素酶报告基因检测来确定miR-518d-5p/519-5p和circRNA8924或CBX8的直接调控作用。结果：研究表明，CC中circRNA8924的表达水平显着高于邻近正常组织（P < 0.001），并且还与肿瘤大小、FIGO分期和子宫肌层浸润相关。 circRNA8924的敲低显着抑制CC细胞SiHa和HeLa的增殖、迁移和侵袭。 miR-518d-5p/519-5p的表达水平与circRNA8924呈负相关，并且circRNA8924通过与miR-518d-5p/519-5p竞争性结合来调节CBX8。结论：CircRNA8924在CC组织中高表达，可以认为是miR-518d-5p/519-5p家族的竞争性内源RNA，促进CC细胞的恶性生物学行为。建议它可以作为CC诊断和疾病进展的新生物标志物，并为靶向治疗提供潜在靶点。 (C) 2018 作者由巴塞尔 S Karger AG 出版
Background/Aims: Circular RNAs (circRNAs) play a significant role in the development and progression of various human cancers. However, the expression and function of circRNAs in cervical cancer (CC) have rarely been explored. The aim of this study was to investigate the biological function of circRNA8924 in CC and elucidate the possible molecular mechanism involved. Methods: Quantitative polymerase chain reaction was used to determine mRNA expression of circRNA8924, miR-518d-5p/519-5p and CBX8 in CC tissues and cells. CBX8 protein expression was measured by Western blotting. The CCK-8 assay was used to evaluate cell proliferation, and the transwell assay to determine cell migration and invasion. The luciferase reporter assay was used to determine the direct regulation of miR-518d-5p/519-5p and circRNA8924 or CBX8 Results: The study demonstrated that the expression level of circRNA8924 in CC was significantly higher than that in the adjacent normal tissues (P < 0.001), and that it was also associated with tumor size, FIGO staging and myometrial invasion. The knockdown of circRNA8924 significantly inhibited the proliferation, migration and invasion of CC cells SiHa and HeLa. The expression level of miR-518d-5p/519-5p was negatively correlated with circRNA8924, and circRNA8924 regulated CBX8 by competitively binding to miR-518d-5p/519-5p. Conclusions: CircRNA8924 is highly expressed in CC tissue and can be considered a competitive endogenous RNA of the miR-518d-5p/519-5p family to promote the malignant biological behavior of CC cells. It is suggested that it may serve as a new biomarker for CC diagnosis and disease progression and provide potential targets for targeted therapy. (C) 2018 The Author(s) Published by S Karger AG, Basel