Immune adaptation to chronic intense exercise training: new microarray evidence.
Immune adaptation to chronic intense exercise training: new microarray evidence.
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DOI：
10.1186/s12864-016-3388-5
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发表时间：
2017-01-05
期刊：
影响因子：
4.4
通讯作者：
Chen P
中科院分区：
文献类型：
--
作者：
Liu D;Wang R;Grant AR;Zhang J;Gordon PM;Wei Y;Chen P
耐力运动训练，特别是大强度训练，对机体免疫系统有很强的影响。然而，运动的免疫调节作用的机制仍不清楚。因此，我们选择调查的变化，在年轻的耐力运动员与健康的久坐不动的对照组相比，使用Affyphase人类基因1.1 ST阵列的血液白细胞的转录谱。使用基于强度的分层贝叶斯方法，然后使用基于逻辑回归的基因集富集方法分析转录组的组差异。我们鉴定了72个在年轻耐力运动员的白细胞转录组中差异表达的显著转录物，与非运动员对照相比，错误发现率（FDR）< 0.05，主要包括编码核糖体蛋白的基因和参与线粒体氧化磷酸化的基因。基因集富集分析确定了三个主要的基因集簇：两个在运动员中上调，包括基因翻译和核糖体蛋白质生产，线粒体氧化磷酸化和生物合成;一个在运动员中转录下调的基因集簇与炎症和免疫活性有关。我们的数据表明，在年轻的健康个体中，剧烈的耐力运动训练（以运动训练为例）可以长期诱导外周血白细胞的转录变化，上调与蛋白质产生和线粒体能量学相关的基因，下调与炎症反应相关的基因。这项研究的结果也为外周血可以作为替代组织来研究运动训练的全身效应的观点提供了支持。本文的在线版本（doi：10.1186/s12864-016-3388-5）包含补充材料，可供授权用户使用。
Endurance exercise training, especially the high-intensity training, exhibits a strong influence on the immune system. However, the mechanisms underpinning the immune-regulatory effect of exercise remain unclear. Consequently, we chose to investigate the alterations in the transcriptional profile of blood leukocytes in young endurance athletes as compared with healthy sedentary controls, using Affymetrix human gene 1.1 ST array. Group differences in the transcriptome were analyzed using Intensity-based Hierarchical Bayes method followed by a Logistic Regression-based gene set enrichment method. We identified 72 significant transcripts differentially expressed in the leukocyte transcriptome of young endurance athletes as compared with non-athlete controls with a false discovery rate (FDR) < 0.05, comprising mainly the genes encoding ribosomal proteins and the genes involved in mitochondrial oxidative phosphorylation. Gene set enrichment analysis identified three major gene set clusters: two were up-regulated in athletes including gene translation and ribosomal protein production, and mitochondria oxidative phosphorylation and biogenesis; one gene set cluster identified as transcriptionally downregulated in athletes was related to inflammation and immune activity. Our data indicates that in young healthy individuals, intense endurance exercise training (exemplifed by athletic training) can chronically induce transcriptional changes in the peripheral blood leukocytes, upregulating genes related to protein production and mitochondrial energetics, and downregulating genes involved in inflammatory response. The findings of the study also provide support for the notion that peripheral blood can be used as a surrogate tissue to study the systemic effect of exercise training. The online version of this article (doi:10.1186/s12864-016-3388-5) contains supplementary material, which is available to authorized users.
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影响因子：
3.7
作者：
Mukherjee K;Edgett BA;Burrows HW;Castro C;Griffin JL;Schwertani AG;Gurd BJ;Funk CD
通讯作者：
Funk CD
影响因子：
12.3
作者：
Gentleman RC;Carey VJ;Bates DM;Bolstad B;Dettling M;Dudoit S;Ellis B;Gautier L;Ge Y;Gentry J;Hornik K;Hothorn T;Huber W;Iacus S;Irizarry R;Leisch F;Li C;Maechler M;Rossini AJ;Sawitzki G;Smith C;Smyth G;Tierney L;Yang JY;Zhang J
通讯作者：
Zhang J
影响因子：
4.8
作者：
Mahoney, DJ;Parise, G;Tarnopolsky, MA
通讯作者：
Tarnopolsky, MA
影响因子：
3.3
作者：
Buettner, Petra;Mosig, Sandy;Mooren, Frank C.
通讯作者：
Mooren, Frank C.
影响因子：
4.4
作者：
Dhabhar, Firdaus S.
通讯作者：
Dhabhar, Firdaus S.