Pararhodobacter marinus sp. nov., a bacterium isolated from marine sediment in the East China Sea and emended description of the genus Pararhodobacter
Pararhodobacter marinus sp. nov., a bacterium isolated from marine sediment in the East China Sea and emended description of the genus Pararhodobacter
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副红细菌 (Pararhodobacter marinus)
DOI：
10.1099/ijsem.0.003627
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发表时间：
2019
影响因子：
2.8
通讯作者：
Shan He
中科院分区：
文献类型：
--
作者：
Weiyan Zhang;Can Chen;Ye Yuan;Dengquan Su;Lijian Ding;Slava S. Epstein;Shan He
从东海舟山岛海洋沉积物中分离到一株革兰氏阴性、棒状、不活动、严格需氧细菌，命名为ZQ420T。菌株zq420t能在10-45℃、0 - 12.0% (w/v) NaCl和pH 5.5-9.0条件下生长。过氧化氢酶和氧化酶活性、硝酸盐还原、H2S生成、淀粉水解、酪蛋白水解、吐温20、吐温40和吐温80均呈阳性。吲哚、甲基红、Voges-Proskauer试验、明胶水解、Tween 60均为阴性。细胞脂肪酸主要为C18: 1ω7c、C16: 0和11-甲基C18: 1ω7c。唯一检测到的呼吸醌是泛醌-10 （Q-10）。极性脂质由二磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油、三种未识别的磷脂、三种未识别的糖脂、两种未识别的氨基脂和两种未识别的脂质组成。DNA G+C含量为64.8 mol%。根据16S rRNA基因序列相似性，菌株zq420t3与以下已验证的菌种（pararhodobacter aggregansD1-19T、Pseudo rhodobacter psych耐受性spamc27389t3和Pseudo rhodobacter collinsensis4-T-34T）的相似性分别为97.9%、96.3%和96.3%。序列相似性较低（< 96.0%）。系统发育分析显示菌株ZQ420TandP。aggregansd1 -19在系统发育树上形成了一个独立的集群。菌株ZQ420TandP之间的平均核苷酸同源值。aggregansd1 -19为79.1%。他们的硅DNA - DNA杂交分析显示，菌株zq420t与p有21.5%的DNA亲缘关系。aggregansD1-19T。根据其表型、化学分类和基因型特征，认为菌株zq420s代表了pararhodobacter属的一个新种，其名称为pararhodobacter marinussp。11月被提议。型应变为ZQ420T（=KCTC 62579T=MCCC 1K03530T）。
A Gram-negative, rod-shaped, non-motile and strictly aerobic bacterium, designated ZQ420T, was isolated from marine sediment sampled on Zhoushan Island located in the East China Sea. Strain ZQ420Twas able to grow at 10–45 °C, 0–12.0 % (w/v) NaCl and pH 5.5–9.0. Catalase and oxidase activities, nitrate reduction, H2S production, hydrolysis of starch, casein, Tween 20, 40 and 80 were positive. Indole, methyl red, Voges–Proskauer test, hydrolysis of gelatin and Tween 60 were negative. The major cellular fatty acids were C18 : 1ω7c, C16 : 0and 11-methyl C18 : 1ω7c. Ubiquinone-10 (Q-10) was the only detected respiratory quinone. The polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, three unidentified phospholipids, three unidentified glycolipids, two unidentified aminolipid and two unidentified lipids. The DNA G+C content was 64.8 mol%. According to 16S rRNA gene sequence similarities, strain ZQ420Tshared 97.9, 96.3 and 96.3 % similarities to the following species with validated namesPararhodobacter aggregansD1-19T,Pseudo rhodobacter psychrotoleransPAMC27389TandPseudo rhodobacter collinsensis4-T-34T, respectively. While sharing lower sequence similarities (<96.0 %) to other type species. Phylogenetic analyses showed that strain ZQ420TandP. aggregansD1-19Tformed an independent cluster in the phylogenetic trees. The average nucleotide identity value between strain ZQ420TandP. aggregansD1-19Twas 79.1 %. Thein silicoDNA–DNA hybridization analysis revealed that strain ZQ420Tshared 21.5 % DNA relatedness withP. aggregansD1-19T. On the basis of its phenotypic, chemotaxonomic and genotypic characteristics, strain ZQ420Tis considered to represent a novel species in the genusPararhodobacter, for which the namePararhodobacter marinussp. nov. is proposed. The type strain is ZQ420T(=KCTC 62579T=MCCC 1K03530T).