Molecular tagging and genetic mapping of the disease resistance gene RppQ to southern corn rust
Molecular tagging and genetic mapping of the disease resistance gene RppQ to southern corn rust
复制标题
DOI：
10.1007/s00122-003-1506-7
复制
发表时间：
2004-03-01
影响因子：
5.4
通讯作者：
Wang, B
中科院分区：
文献类型：
--
作者：
Chen, CX;Wang, ZL;Wang, B
南方玉米锈病（Southern corn rust，SCR）是玉米（Zea mays L.）自交系齐319是一个高抗水稻品种。对抗病亲本齐319的5个F2群体和5个BC 1F 1群体的接种试验和SCR遗传分析结果表明，齐319对SCR的抗性受1对显性抗性基因控制，命名为RppQ。以“齐319 × 340”杂交组合的F-2群体为材料，进行了SSR分析。首先筛选出均匀分布在第10染色体上的20对SSR引物。其中phi 118和phi 041两对引物与SCR抗性连锁。在此基础上，围绕引物phi 118和phi 041的区域，筛选出8对新的SSR引物，并进一步对它们与RppQ的连锁关系进行了检测。结果表明，SSR标记umc 1，318和umc 2，018与RppQ连锁，遗传距离分别为4.76和14.59cM。在RppQ的另一侧，除了标记phi 041和phi 118外，还有一个SSR标记umc 1，293与RppQ连锁，遗传距离为3.78cM。由于5个连锁SSR标记（phi 118、phi 041、umc 1，318、umc 2，018和umc 1，293）均位于第10染色体上，因此RppQ基因也应该位于第10染色体上。为了精细定位RppQ基因，进行了AFLP（扩增片段长度多态性）分析。对54对AFLP引物组合进行了分析，其中E-AGC/M-CAA引物组合扩增产物中的一个AFLP标记AF 1与RppQ基因连锁，遗传距离为3.34 cM。最后将RppQ基因定位在第10染色体短臂上，SSR标记phi 041和AFLP标记AF 1之间的遗传距离分别为2.45和3.34cM。
Southern corn rust (SCR), Puccinia polysora Underw, is a destructive disease in maize (Zea mays L.). Inbred line Qi319 is highly resistant to SCR. Results from the inoculation test and genetic analysis of SCR in five F-2 populations and five BC1F1 populations derived from resistant parent Qi319 clearly indicate that the resistance to SCR in Qi319 is controlled by a single dominant resistant gene, which was named RppQ. Simple sequence repeat (SSR) analysis was carried out in an F-2 population derived from the cross "Qi319x340". Twenty SSR primer pairs evenly distributed on chromosome10 were screened at first. Out of them, two primer pairs, phi118 and phi 041, showed linkage with SCR resistance. Based on this result, eight new SSR primer pairs surrounding the region of primers phi118 and phi 041 were selected and further tested regarding their linkage relation with RppQ. Results indicated that SSR markers umc1,318 and umc 2,018 were linked to RppQ with a genetic distance of 4.76 and 14.59 cM, respectively. On the other side of RppQ, beyond SSR markers phi 041 and phi118, another SSR marker umc1,293 was linked to RppQ with a genetic distance of 3.78 cM. Because the five linkage SSR markers (phi118, phi 041, umc1,318, umc 2,018 and umc1,293) are all located on chromosome 10, the RppQ gene should also be located on chromosome 10. In order to fine map the RppQ gene, AFLP (amplified fragment length polymorphism) analysis was carried out. A total 54 AFLP primer combinations were analyzed; one AFLP marker, AF1, from the amplification products of primer combination E-AGC/M-CAA, showed linkage with the RppQ gene in a genetic distance of 3.34 cM. Finally the RppQ gene was mapped on the short arm of chromosome 10 between SSR markers phi 041 and AFLP marker AF1 with a genetic distance of 2.45 and 3.34 cM respectively.