Phorbol esters down-regulate transcription and translation of the CD4 gene.
Phorbol esters down-regulate transcription and translation of the CD4 gene.
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DOI：
10.4049/jimmunol.146.8.2836
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发表时间：
1991-04
影响因子：
4.4
通讯作者：
S. Neudorf;M. Jones;S. Parker;R. Papes;D. Lattier
中科院分区：
文献类型：
--
作者：
S. Neudorf;M. Jones;S. Parker;R. Papes;D. Lattier
本研究探讨了PMA对CD4分子和基因的生物合成和转录的影响，以确定PMA处理后导致细胞表面CD4分子表达减少的机制。PMA处理的细胞显示CD4分子的生物合成减少，但I类HLA分子的生物合成没有减少。此外，PMA治疗导致CD4 mRNA的稳态水平降低和CD4基因转录的相对速率的抑制。然而，表达转染的CD4 cDNA基因产物的细胞响应于PMA而调节，比表达野生型CD4基因产物的细胞更早地重新表达CD4。这些数据表明，CD 4分子的细胞表面表达可能在蛋白质和基因水平上下调，并且转录的抑制可能会影响CD 4表达的动力学。这些观察结果为HIV影响CD4表达的机制提供了进一步的见解。
This study investigated the effects of PMA on biosynthesis and transcription of the CD4 molecule and gene in order to define mechanisms resulting in reduced cell surface expression of the CD4 molecule after treatment with PMA. Cells treated with PMA showed reduced biosynthesis of the CD4 molecule but not of class I HLA molecules. Furthermore, PMA treatment resulted in reduced steady-state levels of CD4 mRNA and inhibition of the relative rate of transcription of the CD4 gene. Cells expressing transfected CD4 cDNA gene products modulated in response to PMA, however, re-expressed CD4 earlier than cells expressing the product of the wild-type CD4 gene. These data suggest that the cell surface expression of the CD4 molecule is probably down-regulated at the level of the protein, as well as the gene, and that inhibition of transcription may affect the kinetics of CD4 expression. These observations provide further insight into the mechanisms by which HIV affects expression of CD4.