Integration of light and temperature sensing by liquid-liquid phase separation of phytochrome B
Integration of light and temperature sensing by liquid-liquid phase separation of phytochrome B
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DOI：
10.1016/j.molcel.2022.05.026
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发表时间：
2022-08-18
期刊：
影响因子：
16
通讯作者：
Zhong, Shangwei
中科院分区：
文献类型：
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作者：
Chen, Di;Lyu, Mohan;Zhong, Shangwei
植物中的光和温度由一种共同的受体光敏色素B（phyB）感知。phyB如何区分这些信号仍然难以捉摸。在这里，我们报告说，phyB自发地进行相分离组装液体状液滴。这种能力是由其C末端通过自缔合驱动的，而本质上无序的N末端延伸（NTE）作为相分离的生物物理调节剂起作用。光暴露触发构象变化，随后改变phyB冷凝物组装，而温度感觉是直接介导的NTE调节phyB液滴的相行为。多个信号组分被选择性地掺入phyB液滴中以形成浓缩的微反应器，从而允许通过相变对phyB信号活性进行开关样控制。因此，光和温度的线索分别读出phyB通过变构变化和自发相分离，分别。我们提供了一个概念框架，显示如何不同的，但高度相关的物理信号的解释和排序的一个受体。
Light and temperature in plants are perceived by a common receptor, phytochrome B (phyB). How phyB distinguishes these signals remains elusive. Here, we report that phyB spontaneously undergoes phase separation to assemble liquid-like droplets. This capacity is driven by its C terminus through self-association, whereas the intrinsically disordered N-terminal extension (NTE) functions as a biophysical modulator of phase separation. Light exposure triggers a conformational change to subsequently alter phyB condensate assembly, while temperature sensation is directly mediated by the NTE to modulate the phase behavior of phyB droplets. Multiple signaling components are selectively incorporated into phyB droplets to form concentrated microreactors, allowing switch-like control of phyB signaling activity through phase transitions. Therefore, light and temperature cues are separately read out by phyB via allosteric changes and spontaneous phase separation, respectively. We provide a conceptual framework showing how the distinct but highly correlated physical signals are interpreted and sorted by one receptor.