Effect of n-3 fatty acids on the expression of inflammatory genes in THP-1 macrophages.
Effect of n-3 fatty acids on the expression of inflammatory genes in THP-1 macrophages.
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DOI：
10.1186/s12944-016-0241-4
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发表时间：
2016-04-05
影响因子：
4.5
通讯作者：
Vohl MC
中科院分区：
文献类型：
--
作者：
Allam-Ndoul B;Guénard F;Barbier O;Vohl MC
不受控制的炎症参与炎性疾病的发展。已经报道了属于n-3家族的多不饱和脂肪酸如二十碳五烯酸（EPA）和二十二碳六烯酸（DHA）对炎症的有益作用。本研究探讨了EPA、DHA和EPA + DHA混合物对未受刺激的培养的THP 1巨噬细胞中与炎症相关的10个基因（AKT 1、MAPK、NF κ B、TNFA、IL 1 Β、MCP 1、ALOX 5、PTGS 2、MG ST 1和NOS 2）表达的基础作用。将细胞与n-3 PUFA（50 μM和10 μM EPA、DHA、EPA + DHA）孵育24小时。通过实时PCR分析炎性基因的表达水平。50 μM、10 μM EPA和50 μM EPA + DHA可降低NF-κB通路相关基因（MAPK、AKT 1和NF κ B）的表达。用50 μM、10 μM EPA、50 μM DHA和EPA + DHA处理降低了细胞因子基因IL 1 Β和MCP 1的表达水平。50 μM、10 μM EPA、DHA和50 μM EPA + DHA均能降低TNFA的表达。参与脂肪酸代谢的两个基因（PTGS 2和ALOX 5）也受到n-3 FA的调节。当两种浓度的EPA、50 μM DHA和EPA + DHA抑制ALOX 5表达时，50 μM DHA和EPA + DHA抑制PTGS 2表达。最后，研究了n-3脂肪酸在参与氧化应激的基因中的作用。50 μM的每种脂肪酸增加MGST 1表达。EPA和50 μM DHA均能降低NOS 2的表达。EPA似乎比DHA和EPA + DHA更有效地调节选定的炎症基因的表达水平。50 μM浓度总体上比10 μM更有效。
Uncontrolled inflammation participates in the development of inflammatory diseases. Beneficial effects of polyunsaturated fatty acids belonging to the n-3 family such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on inflammation have been reported. The present study investigates the basal effects of EPA, DHA and a mixture EPA + DHA on the expression of 10 genes (AKT1, MAPK, NFKB, TNFA, IL1Β, MCP1, ALOX5, PTGS2, MGST1and NOS2) related to inflammation in unstimulated cultured THP1 macrophages. Cells were incubated for 24 h with n-3 PUFAs (50 μM and 10 μM EPA, DHA, EPA + DHA). Expression levels of inflammatory genes were analyzed by real-time PCR. 50 μM, 10 μM EPA and 50 μM EPA + DHA decreased the expression of genes involved in the NF-κB pathway (MAPK, AKT1, and NFKB). Treatment with 50 μM, 10 μM EPA, 50 μM DHA and EPA + DHA decreased expression levels of cytokines genes IL1Β and MCP1. TNFA expression was decreased by 50 μM, 10 μM of EPA, DHA and with 50 μM EPA + DHA. Two genes involved in the fatty acid metabolism (PTGS2 and ALOX5) were also modulated by the n-3 FAs. 50 μM of DHA and EPA + DHA inhibited PTGS2 expression when the two concentrations of EPA, 50 μM DHA and EPA + DHA inhibited ALOX5 expression. Finally, the effects of n-3 FAs were studied among genes involved in the oxidative stress. 50 μM of each fatty acid increased MGST1 expression. Both concentration of EPA and 50 μM DHA decreased NOS2 expression. EPA seems to be more effective than DHA and EPA + DHA in modulating expression levels of selected inflammatory genes. The concentration of 50 μM was globally more effective than 10 μM.