Identification of Light-Independent Anthocyanin Biosynthesis Mutants Induced by Ethyl Methane Sulfonate in Turnip "Tsuda" (Brassica rapa).
Identification of Light-Independent Anthocyanin Biosynthesis Mutants Induced by Ethyl Methane Sulfonate in Turnip "Tsuda" (Brassica rapa).
复制标题
甲磺酸乙酯诱导的萝卜“Tsuda”（Brassica rapa）中不依赖于光的花青素生物合成突变体的鉴定
DOI：
10.3390/ijms18071288
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发表时间：
2017-06-22
影响因子：
5.6
通讯作者：
Wang Y
中科院分区：
文献类型：
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作者：
Yang JF;Chen YZ;Kawabata S;Li YH;Wang Y
萝卜“Tsuda”(Brassica Rapa)紫色品种膨大贮藏根的表皮以一种光依赖的方式积累花青素，特别是对UV-A光的响应，其机制尚不清楚。在本研究中，我们用0.5%(v/v)的乙基甲烷磺酸盐(EMS)诱变了15,000颗种子，获得了14个膨大贮藏根的表皮花青素产生异常的突变体。这些突变体可分为两类：一类是在黑暗条件下贮藏根的表皮即使在地下部分也有结构性花青素积累的红色突变体，另一类是在阳光照射下的地上部分贮藏根的表皮不积累花青素的白色突变体。测试交叉分析表明，W9、W68、W204、R15、R21、R30和R57含有不同的突变，导致了它们的表型变异。对4个目标突变体(W9、W68、W204和R15)的进一步遗传分析表明，每个突变体都受不同的隐性基因控制。有趣的是，在四个目标突变体中，花青素生物合成基因的表达谱，包括结构基因和调控基因，与它们在贮藏根表皮中的花青素水平一致。我们提出导致这些突变的潜在基因可能是萝卜花青素生物合成途径的上游因子，这为进一步研究光诱导的花青素积累机制提供了资源。
The epidermis of swollen storage roots in purple cultivars of turnip “Tsuda” (Brassica rapa) accumulates anthocyanin in a light-dependent manner, especially in response to UV-A light, of which the mechanism is unclear. In this study, we mutagenized 15,000 seeds by 0.5% (v/v) ethyl methane sulfonate (EMS) and obtained 14 mutants with abnormal anthocyanin production in their epidermis of swollen storage roots. These mutants were classified into two groups: the red mutants with constitutive anthocyanin accumulation in their epidermis of storage roots even in underground parts in darkness and the white mutants without anthocyanin accumulation in the epidermis of storage roots in aboveground parts exposed to sunlight. Test cross analysis demonstrated that w9, w68, w204, r15, r21, r30 and r57 contained different mutations responsible for their phenotypic variations. Further genetic analysis of four target mutants (w9, w68, w204 and r15) indicated that each of them was controlled by a different recessive gene. Intriguingly, the expression profiles of anthocyanin biosynthesis genes, including structural and regulatory genes, coincided with their anthocyanin levels in the epidermis of storage roots in the four target mutants. We proposed that potential genes responsible for the mutations should be upstream factors of the anthocyanin biosynthesis pathway in turnips, which provided resources to further investigate the mechanisms of light-induced anthocyanin accumulation.