Effects of Exogenous Application of Plant Growth Regulators (SNP and GA3) on Phytoextraction by Switchgrass (Panicum virgatum L.) Grown in Lead (Pb) Contaminated Soil
Effects of Exogenous Application of Plant Growth Regulators (SNP and GA3) on Phytoextraction by Switchgrass (Panicum virgatum L.) Grown in Lead (Pb) Contaminated Soil
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DOI：
10.3390/su131910866
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发表时间：
2021-10-01
期刊：
影响因子：
3.9
通讯作者：
Greipsson, Sigurdur
中科院分区：
文献类型：
--
作者：
Beavers, Adrianne;Koether, Marina;Greipsson, Sigurdur
土壤铅污染是一个重大的环境和公众健康风险。柳枝稷是第二代生物燃料作物，具有长期修复和植物提取铅污染土壤的潜力。我们评估了植物生长调节剂和土壤杀真菌剂和螯合剂的协调叶面应用的功效，以优化植物提取。植物在受控条件下在土壤培养物中生长。首先，在多个浓度下评估三种外源性一氧化氮（NO）供体：（1）S-亚硝基-N-乙酰青霉胺（SNAP）;（2）硝普钠（SNP）;和（3）S-亚硝基谷胱甘肽（GSNO）。第二，SNP（0.5 μ M）的影响，进一步检查与模型螯合EDTA和土壤杀菌剂丙环唑。第三，用EDTA和丙环唑检查SNP和赤霉素（GA 3）的组合叶面施用。丙环唑（一种广谱杀菌剂）的土壤应用减少AMF的殖民化，并允许更大的铅植物提取。SNP的叶面施用导致类似浓度的铅（根和叶）的植物，被挑战与螯合物和土壤杀菌剂。SNP和GA 3的组合叶面施用导致植物叶片中的平均Pb浓度（243 mg kg（-1））显著高于对照植物（182 mg kg（-1））和仅用GA 3处理的植物（202 mg kg（-1））。SNP和GA 3的组合叶面施用导致最大的植物提取效率，因此可能会提高植物提取铅污染土壤中生长的柳枝稷。
Soil lead (Pb) contamination is a major environmental and public health risk. Switchgrass (Panicum virgatum), a second-generation biofuel crop, is potentially useful for the long-term phytoremediation and phytoextraction of Pb contaminated soils. We evaluated the efficacy of a coordinated foliar application of plant growth regulators and soil fungicide and a chelator in order to optimize phytoextraction. Plants were grown in soil culture under controlled conditions. First, three exogenous nitric oxide (NO) donors were evaluated at multiple concentrations: (1) S-nitroso-N-acetylpenicillamine (SNAP); (2) sodium nitroprusside (SNP); and (3) S-nitrosoglutathione (GSNO). Second, the effect of SNP (0.5 mu M) was examined further with the model chelate EDTA and the soil fungicide propicanazole. Third, a combined foliar application of SNP and gibberellic acid (GA3) was examined with EDTA and propicanazole. The soil application of propiconazole (a broad-spectrum fungicides) reduced AMF colonization and allowed greater Pb phytoextraction. The foliar application of SNP resulted in similar concentrations of Pb (roots and foliage) to plants that were challenged with chelates and soil fungicides. The combined foliar application of SNP and GA3 resulted in significantly greater average Pb concentration (243 mg kg(-1)) in plant foliage in comparison to control plants (182 mg kg(-1)) and plants treated with GA3 alone (202 mg kg(-1)). The combined foliar application of SNP and GA3 resulted in the greatest phytoextraction efficiency and could therefore potentially improve phytoextraction by switchgrass grown in Pb contaminated soils.