Meta-Analysis and Multiomics of a Chromosome Segment Substitution Line Reveal Candidate Genes Associated with Seed Hardness in Soybean.
Meta-Analysis and Multiomics of a Chromosome Segment Substitution Line Reveal Candidate Genes Associated with Seed Hardness in Soybean.
复制标题
DOI：
10.1021/acs.jafc.3c03950
复制
发表时间：
2023-10
影响因子：
6.1
通讯作者：
Ning Wang;Shaowei Feng;Xuntong Ma;Qingshan Chen;Chunyan Liu;Zhaoming Qi
中科院分区：
文献类型：
--
作者：
Ning Wang;Shaowei Feng;Xuntong Ma;Qingshan Chen;Chunyan Liu;Zhaoming Qi
大豆种子硬度是影响大豆种植、营养品质和采后加工的关键性状，但其遗传和分子机制仍有待阐明。利用Meta分析方法检测了17个大豆种子硬度的元数量性状位点。然后，我们确定了一个硬种子的染色体片段置换线，R75，从硬种子的野生种质在四个元QTL间隔的片段。种皮超微结构的观察表明，厚栅栏组织在R75比其软籽轮回亲本。R75及其轮回亲本的转录组学和蛋白质组学研究揭示了多个与种子硬度相关的候选基因。57个位于纯合引入片段上，26个位于元QTL间隔中，一个位于两者中（Glyma.02G268600）。五个最初的候选人被选为KASP标记开发的基础上，他们的预测功能和非同义SNP。在染色体片段代换系群体中，标记对非硬系的选择效率高达90%，对硬系的选择效率高达43%。
Soybean seed hardness is a key trait that influences planting, nutritional quality, and postharvest processing, but its genetic and molecular mechanisms remain to be clarified. We used meta-analysis to detect 17 meta-quantitative trait locus (QTLs) for soybean seed hardness. We then identified a hard-seeded chromosome segment substitution line, R75, with fragments introduced from hard-seeded wild germplasm in four of the meta-QTL intervals. Observations of the seed coat ultrastructure revealed thicker palisade tissue in R75 than in its soft-seeded recurrent parent. Transcriptomics and proteomics of R75 and its recurrent parent revealed multiple candidate genes associated with seed hardness. Fifty-seven were located on homozygous introduced fragments, 26 in meta-QTL intervals, and one in both (Glyma.02G268600). Five initial candidates were selected for KASP marker development on the basis of their predicted functions and nonsynonymous SNPs. The selection efficiency of the markers was as high as 90% for nonhard lines and 43% for hard lines in the chromosome segment substitution line (CSSL) population.