Sertoli cell differentiation on basement membrane is mediated by the c-fos protooncogene.
Sertoli cell differentiation on basement membrane is mediated by the c-fos protooncogene.
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基底膜上支持细胞的分化是由 c-fos 原癌基因介导的。
DOI：
10.1073/pnas.91.15.7027
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发表时间：
1994
影响因子：
11.1
通讯作者：
Dym,M
中科院分区：
文献类型：
--
作者：
Papadopoulos,V;Dym,M
当睾丸中生精上皮的支持细胞在Matrigel（重建基底膜）、层粘连蛋白或一种具有生物活性的层粘连蛋白衍生肽（YIGSR、SIKVAV或RGD）上培养时，它们表现出形态上的显著变化，伴随着蛋白分泌和基因表达的变化，包括c-fos mRNA的快速诱导刺激。为了研究c-fos在支持细胞在细胞外基质上的粘附、铺展和分化中的作用，我们构建了c-fos硫代磷酸寡核苷酸（ODNs）的正、反义核酸。在基质胶或层粘连蛋白上，在存在c-fos反义ODN（30微克/毫升）的情况下培养的10-20个细胞的小团块中的支持细胞在最初的10-20小时内不扩散。在补充有对c-fos有感觉的ODN（30微克/ml）或没有ODN的培养基中培养的细胞在30分钟至1小时内铺展，12小时后建立单层。此外，孵育支持细胞与反义ODNs的c-fos蛋白水平显着降低，而治疗与正义的c-fos的ODNs几乎不影响c-fos蛋白的表达。这些数据表明，c-fos可能介导的事件涉及支持细胞的附着和扩散后，细胞与细胞外基质的接触。
When Sertoli cells of the seminiferous epithelium in the testis were cultured on Matrigel (a reconstituted basement membrane), laminin, or one of the biologically active laminin-derived peptides (YIGSR, SIKVAV, or RGD), they exhibited dramatic changes in morphology accompanied by changes in protein secretion and gene expression, including a rapidly induced stimulation of c-fos mRNA. To examine the role of c-fos in Sertoli cell attachment, spreading, and differentiation on extracellular matrix, we constructed sense and antisense c-fos phosphorothioate oligodeoxynucleotides (ODNs). Sertoli cells, in small clumps of 10-20 cells, cultured on Matrigel or laminin in the presence of ODNs antisense to c-fos (30 micrograms/ml) did not spread for the first 10-20 hr. After that time, normal spreading occurred, probably as a result of ODN degradation. Cells cultured in medium supplemented with ODNs sense to c-fos (30 micrograms/ml), or without ODNs, spread within 30 min to 1 hr, and after 12 hr a monolayer was established. Furthermore, incubation of Sertoli cells with ODNs antisense to c-fos resulted in a significant reduction of c-fos protein levels, whereas treatment with ODNs sense to c-fos barely affected c-fos protein expression. These data indicate that c-fos may mediate the events involved in Sertoli cell attachment and spreading upon contact of the cells with extracellular matrix.