Surgical anatomy of the ovine sural nerve for facial nerve regeneration and reconstruction research
Surgical anatomy of the ovine sural nerve for facial nerve regeneration and reconstruction research
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DOI：
10.1038/s41598-019-46661-3
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发表时间：
2019-07-22
影响因子：
4.6
通讯作者：
Enkhbaatar, Perenlei
中科院分区：
文献类型：
--
作者：
Niimi, Yosuke;Fukuda, Satoshi;Enkhbaatar, Perenlei
缺乏临床相关的动物模型用于面神经重建的研究是具有挑战性的。在这项研究中，我们调查了绵羊腓肠神经的外科解剖作为一个潜在的候选人，面神经重建，并进行了组织学定量分析比较颊分支（BB）的面神经使用尸体和麻醉羊。绵羊腓肠神经沿着短隐静脉下行至小腿。腓肠神经长度为14.3 ± 0.5 cm。外踝后缘至腓肠神经的距离为7.8 ± 1.8 mm。腓肠神经的平均有髓纤维数明显低于BB（2，311 ± 381 vs. 5，022 +/- 433，分别。p = 0.003）。腓肠神经的神经束数量也显著低于BB（分别为10.5 +/- 1.7 vs. 21.3 +/- 2.7）。p = 0.007）。在手术显微镜下，将腓肠神经端端吻合移植到BB上。绵羊腓肠神经的外科解剖和神经束的数量与人类报告的相似。结果表明，腓肠神经可以成功地用于面神经重建研究在临床相关的绵羊模型。
The lack of a clinically relevant animal models for research in facial nerve reconstruction is challenging. In this study, we investigated the surgical anatomy of the ovine sural nerve as a potential candidate for facial nerve reconstruction, and performed its histological quantitative analysis in comparison to the buccal branch (BB) of the facial nerve using cadaver and anesthetized sheep. The ovine sural nerve descended to the lower leg along the short saphenous vein. The length of the sural nerve was 14.3 +/- 0.5 cm. The distance from the posterior edge of the lateral malleolus to the sural nerve was 7.8 +/- 1.8 mm. The mean number of myelinated fibers in the sural nerve was significantly lower than that of the BB (2,311 +/- 381vs. 5,022 +/- 433, respectively. p = 0.003). The number of fascicles in the sural nerve was also significantly lower than in the BB (10.5 +/- 1.7 vs. 21.3 +/- 2.7, respectively. p = 0.007). The sural nerve was grafted to the BB with end-to-end neurorrhaphy under surgical microscopy in cadaver sheep. The surgical anatomy and the number of fascicles of the ovine sural nerve were similar of those reported in humans. The results suggest that the sural nerve can be successfully used for facial nerve reconstruction research in a clinically relevant ovine model.