Colonization of a Newly Constructed Commercial Chicken Further Processing Plant with Listeria monocytogenes
Colonization of a Newly Constructed Commercial Chicken Further Processing Plant with Listeria monocytogenes
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DOI：
10.4315/0362-028x-73.2.286
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发表时间：
2010-02-01
影响因子：
2
通讯作者：
Ladely, Scott R.
中科院分区：
文献类型：
--
作者：
Berrang, Mark E.;Meinersmann, Richard J.;Ladely, Scott R.
本研究旨在确定新建的鸡肉深加工工厂中单核细胞增生李斯特菌的潜在来源，并记录该病原体在该工厂的最终定植情况。为了确定植物的定植状况，在生产班次后和清理班次后，大约每个月采样一次地沟，持续21个月。单核细胞增生乳杆菌的潜在来源包括传入的生肉。进来的新鲜空气和人员。附近的环境和社区样本也进行了检查。所有检测到的单核增生乳杆菌都进行了基于DNA序列的分型。在开始加工操作之前，在工厂中未检测到单核细胞增生乳杆菌。在4个月内，在清洁和消毒操作前后，在地漏中检测到几种亚型的单核细胞增生乳杆菌。在与工厂员工有关的空气过滤器上未检测到单核细胞增生乳杆菌。或者附近的折扣购物中心。在植物附近的一条自然溪流中检测到1种单核增生乳杆菌；然而，这种亚型从未在植物体内检测到。在进行进一步加工的生肉中检测到8种单核细胞增生乳杆菌亚型：其中一种生肉亚型与在4个不同的排水沟中清洗8次后恢复的持久性排水沟亚型难以区分。家禽深加工工厂很可能成为单增李斯特菌的定植地，原料产品是该菌对工厂的重要来源。
This Study was undertaken to determine potential Sources of Listeria monocytogenes in a newly constructed chicken further processing plant and document the eventual colonization of the facility by this pathogen. To ascertain the colonization status of the plant, floor drains were sampled after a production shift and again after a cleanup Shift On roughly a monthly basis for 21 months. Potential sources of L. monocytogenes to the plant included incoming raw meat. incoming fresh air, and personnel. Nearby environment and community samples were also examined. All L. monocytogenes detected were subjected to DNA sequence-based subtyping. L. monocytogenes was not detected in the plant before the commencement of processing operations. Within 4 months, several subtypes of L. monocytogenes were detected in floor drains, both before and after cleaning and sanitizing operations. No L. monocytogenes was detected on filters for incoming air, samples associated with plant employees. or a nearby discount shopping center. One subtype of L. monocytogenes was detected in a natural stream near the plant; however, this subtype was never detected inside the plant. Eight subtypes of L. monocytogenes were detected in raw meat staged for further processing: one of the raw meat subtypes was indistinguishable from a persistent drain subtype recovered after cleaning on eight occasions in four different drains. Poultry further processing plants are likely to become colonized with L. monocytogenes raw product is an important source of the organism to the plant.