Next-Generation Sequencing of an 88-Year-Old Specimen of the Poorly Known Species Liagora japonica (Nemaliales, Rhodophyta) Supports the Recognition of Otohimella gen. nov.
Next-Generation Sequencing of an 88-Year-Old Specimen of the Poorly Known Species Liagora japonica (Nemaliales, Rhodophyta) Supports the Recognition of Otohimella gen. nov.
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对众所周知的Liagora Japonica（Nemaliales，Rhodophyta）的88年历史标本的下一代测序支持了Otohimella Gen的识别。十一月。
DOI：
10.1371/journal.pone.0158944
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Nozaki H
中科院分区：
文献类型：
--
作者：
Suzuki M;Segawa T;Mori H;Akiyoshi A;Ootsuki R;Kurihara A;Sakayama H;Kitayama T;Abe T;Kogame K;Kawai H;Nozaki H
Liagora japonica 是分布于日本温带地区的一种红色藻类。自 1927 年以来，该物种就没有在日本太平洋沿岸的模式产地被采集到，并且似乎已经在该地区灭绝。为了对日本海藻进行分子表征，我们从 1927 年收集的日本海藻拓扑型材料中提取了 DNA，使用 Illumina 下一代测序分析了 7 个基因，并将这些数据与从日本海沿岸收集的类似红藻的现代样本序列进行了比较。现代样本和历史样本（包括选型和拓扑型）的形态学和分子数据表明，来自日本太平洋和日本海沿岸的样本应被视为单一物种，并且 L. japonica 在系统发育上与 Liagora 属分开。根据系统发育结果和生殖结构检查，我们提出 Otohimella japonica gen.等梳子。 11月，其形态特征为弥漫性果孢子体、不分裂的果孢子囊和仅从支持细胞上的皮质细胞起始的总苞丝。
Liagora japonica is a red algal species distributed in temperate regions of Japan. This species has not been collected from its type locality on the Pacific coast of Japan since 1927 and seems to have become extinct in this area. For molecular characterization of L. japonica, we extracted DNA from the topotype material of L. japonica collected in 1927, analyzed seven genes using Illumina next-generation sequencing, and compared these data with sequences from modern samples of similar red algae collected from the Japan Sea coast of Japan. Both morphological and molecular data from modern samples and historical specimens (including the lectotype and topotype) suggest that the specimens from the Pacific and Japan Sea coasts of Japan should be treated as a single species, and that L. japonica is phylogenetically separated from the genus Liagora. Based on the phylogenetic results and examination of reproductive structures, we propose Otohimella japonica gen. et comb. nov., characterized morphologically by diffuse carposporophytes, undivided carposporangia, and involucral filaments initiated only from the cortical cell on the supporting cell.