Combinatorial action of NF-Y and TALE at embryonic enhancers defines distinct gene expression programs during zygotic genome activation in zebrafish.
Combinatorial action of NF-Y and TALE at embryonic enhancers defines distinct gene expression programs during zygotic genome activation in zebrafish.
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DOI：
10.1016/j.ydbio.2019.12.003
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发表时间：
2019-12
影响因子：
2.7
通讯作者：
W. Stanney;Franck Ladam;I. Donaldson;Teagan J. Parsons;R. Maehr;N. Bobola;C. Sagerström
中科院分区：
文献类型：
--
作者：
W. Stanney;Franck Ladam;I. Donaldson;Teagan J. Parsons;R. Maehr;N. Bobola;C. Sagerström
动物胚胎发生是由母体因素启动的，但合子基因组激活(ZGA)将调控控制转移到囊胚期的胚胎。ZGA被认为是由母体提供的转录因子(TF)介导的，但在脊椎动物中很少发现这样的转录因子。在这里，我们报道了与ZGA中已经存在的发育控制基因相关的斑马鱼基因组元件的NF-Y和TALL转录因子。特别是，NF-Y和TALE的共同调节与参与转录控制的广泛作用的基因有关，而NF-Y或TALE的调节定义了特定发育过程中的基因，因此，NF-Y控制纤毛基因表达程序，而TALE控制HOX基因的表达。我们还证明了核因子-Y和故事占据的基因组元件在胚胎发育过程中作为增强剂发挥作用。我们的结论是，在发育促进剂中联合使用NF-Y和TALE可以在斑马鱼ZGA中建立不同的基因表达程序。
Animal embryogenesis is initiated by maternal factors, but zygotic genome activation (ZGA) shifts regulatory control to the embryo during blastula stages. ZGA is thought to be mediated by maternally provided transcription factors (TFs), but few such TFs have been identified in vertebrates. Here we report that NF–Y and TALE TFs bind zebrafish genomic elements associated with developmental control genes already at ZGA. In particular, co-regulation by NF–Y and TALE is associated with broadly acting genes involved in transcriptional control, while regulation by either NF–Y or TALE defines genes in specific developmental processes, such that NF–Y controls a cilia gene expression program while TALE controls expression ofhoxgenes. We also demonstrate that NF–Y and TALE-occupied genomic elements function as enhancers during embryogenesis. We conclude that combinatorial use of NF–Y and TALE at developmental enhancers permits the establishment of distinct gene expression programs at zebrafish ZGA.