Endotoxin tolerance in rats:: Expression of TNF-α, IL-6, IL-10, VCAM-1 and HSP 70 in lung and liver during endotoxin shock
Endotoxin tolerance in rats:: Expression of TNF-α, IL-6, IL-10, VCAM-1 and HSP 70 in lung and liver during endotoxin shock
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DOI：
10.1006/cyto.1998.0490
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发表时间：
1999-10-01
期刊：
影响因子：
3.8
通讯作者：
Schade, FU
中科院分区：
文献类型：
--
作者：
Flohé, S;Fernández, ED;Schade, FU
内毒素可诱导对其自身病理作用的耐受状态，通常称为内毒素耐受。已发现这种现象与细胞因子如TNF-α、TL-1、IL-6和IL;-10的血清水平降低有关。在本研究中，通过静脉注射后半定量 RT-PCR 的方式测定了 LPS 的肺和肝脏中 TNF-α、IL-6、IL-10、粘附分子 VCAM-1 和热休克蛋白 70 的表达；耐受大鼠和幼稚大鼠。脂多糖注射；注射LPS后，肺和肝中诱导TNFα、IL-6、IL-10、HSP 70和VCAM-1。与不耐受内毒素的对照组相比，内毒素耐受大鼠的肝脏和肺中 TNF-α 和 IL-6 在 LPS 处理后仅被诱导至较低程度。耐受内毒素大鼠的肺中 LPS 诱导的 IL-10 表达也略有减弱，但在肝脏中，耐受和非耐受动物之间没有观察到差异。全身 LPS 处理后，肝脏和肺中 HSP 70 和 VCAM-1 表达。然而，耐受性和未治疗对照的诱导程度是相同的。所提供的数据表明，内毒素耐受性通过体内肺和肝中细胞因子表达的减少来反映。另一方面，粘附分子VCAM-1和应激蛋白HSP 70的表达水平似乎并未因内毒素耐受性而改变。 1999年学术出版社。
Endotoxin can induce a state of tolerance against its own pathological effects, commonly referred to as endotoxin tolerance. This phenomenon has been found to be associated with reduced serum levels of cytokines such as TNF-alpha, TL-I, IL-6 and IL;-10. In the present study the expression of TNF-alpha, IL-6, IL-10, the adhesion molecule VCAM-1 and the heat shock protein 70 was determined in vivo in lung and liver of LPS;tolerant and naive rats by means of semiquantitative RT-PCR after i.v. LPS injection; TNF alpha, IL-6, IL-10, HSP 70 and VCAM-1 were induced in lung and liver after LPS injection. In liver and lung of endotoxin-tolerant rats TNF-alpha and IL-6 mere induced to a lower degree after LPS treatment when compared to non-tolerant controls, The LPS-induced IL-10 expression was also slightly attenuated in the lung of tolerant rats, but in the liver no differences between tolerant and non-tolerant animals were observed. HSP 70 and VCAM-1 were expressed after systemic LPS treatment in liver and lung. The degree of induction, however, was the same in tolerant and untreated controls. The presented data show that endotoxin tolerance is reflected by a reduced cytokine expression in lung and liver in vivo. On the other hand, levels of expression of the adhesion molecule VCAM-1 and the stress protein HSP 70 do not appear to be changed by endotoxin tolerance. In 1999 Academic Press.