Functional analysis of rice DREB1/CBF-type transcription factors involved in cold-responsive gene expression in transgenic rice
Functional analysis of rice DREB1/CBF-type transcription factors involved in cold-responsive gene expression in transgenic rice
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DOI：
10.1093/pcp/pci230
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发表时间：
2006-01-01
影响因子：
4.9
通讯作者：
Yamaguchi-Shinozaki, K
中科院分区：
文献类型：
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作者：
Ito, Y;Katsura, K;Yamaguchi-Shinozaki, K
转录因子脱水响应元件结合蛋白1 s（DREB 1 s）/C重复序列结合因子（CBF）特异性地与DRE/CRT顺式作用元件相互作用，并控制拟南芥中许多胁迫诱导基因的表达。水稻DREB 1同源基因OsDREB 1A和OsDREB 1B受冷胁迫诱导，DREB 1或OsDREB 1的过表达诱导了转基因拟南芥中胁迫响应基因的强表达，从而提高了对高盐和冷冻胁迫的耐受性。在这项研究中，我们获得了过量表达OsDREB 1或DREB 1基因的转基因水稻植株。这些转基因水稻植株不仅在正常生长条件下表现出生长迟缓，而且与过表达OsDREB 1或DREB 1的转基因拟南芥植株一样，对干旱、高盐和低温胁迫的耐受性也有所提高。我们还检测到转基因水稻中的游离脯氨酸和各种可溶性糖的含量升高，如在转基因拟南芥植物。我们利用基因芯片和RNA凝胶印迹分析技术鉴定了OsDREB 1A在转基因水稻中的胁迫诱导基因。这些基因编码的蛋白质被认为在植物的胁迫耐受性中起作用。这些结果表明，DREB 1/CBF低温反应途径在水稻中是保守的，DREB 1类基因在提高包括水稻在内的各种转基因植物对环境胁迫的耐受性方面是非常有用的。
The transcription factors dehydration-responsive element-binding protein 1s (DREB1s)/C-repeat-binding factors (CBFs) specifically interact with the DRE/CRT cis-acting element and control the expression of many stress-inducible genes in Arabidopsis. The genes for DREB1 orthologs, OsDREB1A and OsDREB1B from rice, are induced by cold stress, and overexpression of DREB1 or OsDREB1 induced strong expression of stress-responsive genes in transgenic Arabidopsis plants, resulting in increased tolerance to high-salt and freezing stresses. In this study, we generated transgenic rice plants overexpressing the OsDREB1 or DREB1 genes. These transgenic rice plants showed not only growth retardation under normal growth conditions but also improved tolerance to drought, high-salt and low-temperature stresses like the transgenic Arabidopsis plants overexpressing OsDREB1 or DREB1. We also detected elevated contents of osmoprotectants such as free proline and various soluble sugars in the transgenic rice as in the transgenic Arabidopsis plants. We identified target stress-inducible genes of OsDREB1A in the transgenic rice using microarray and RNA gel blot analyses. These genes encode proteins that are thought to function in stress tolerance in the plants. These results indicate that the DREB1/CBF cold-responsive pathway is conserved in rice and the DREB1-type genes are quite useful for improvement of stress tolerance to environmental stresses in various kinds of transgenic plants including rice.