Physical and genetic map of the Clostridium acetobutylicum ATCC 824 chromosome
Physical and genetic map of the Clostridium acetobutylicum ATCC 824 chromosome
复制标题
DOI：
10.1128/jb.179.23.7426-7434.1997
复制
发表时间：
1997-12-01
影响因子：
3.2
通讯作者：
Soucaille, P
中科院分区：
文献类型：
--
作者：
Cornillot, E;Croux, C;Soucaille, P
构建了丙酮丁醇梭菌ATCC 824染色体的物理和遗传图谱。通过一维和二维脉冲场凝胶电泳（PFGE）对38个限制性酶切位点进行排序，并使用基于CeuI酶的原始策略和通过在CeuI位点两侧与rrs（16S RNA）和3' rrl（23S RNA）探针杂交进行间接末端标记，创建了CeuI、CeuI和SstII的宏观限制性酶切图谱。圆形染色体的大小估计为4.15 Mb，物理图谱的平均分辨率为110 kb。染色体含有11个rrn基因座，其位于44%的染色体上，关于复制起点的假定位置，其转录方向不同。除了这11个rrn操纵子外，通过与来自C.丙酮丁醇梭菌和各种其它梭菌作为探针。遗传图谱的构建。将丙酮丁醇芽孢杆菌与迄今为止表征的三种其他内孢子形成细菌的内孢子形成细菌进行比较：枯草芽孢杆菌、拜氏梭菌和产气荚膜梭菌。巧合的是，C.丙酮丁醇与B的相似性更高。枯草芽孢杆菌比梭状芽孢杆菌的。
A physical and genetic map of the Clostridium acetobutylicum ATCC 824 chromosome was constructed. The macrorestriction map for CeuI, EagI, and SstII ws created by ordering the 38 restriction sties by one- and two-dimensional pulsed-filed gel electrophoresis (PFGE) and by using an original strategy based on the CeuI enzyme and indirect end labelling by hybridization on both sides of the CeuI sites with rrs (16S RNA) and 3' rrl (23S RNA) probes. The circular chromosome was estimated to be 4.15 Mb in size, and the average resolution of the physical map is 110 kb. The chromosome contains 11 rrn loci, which are localized on 44% of the chromosome in a divergent transcriptional orientation regarding the presumed location of the replication origin. In addition to these 11 rrn operons, a total of 40 identified genes were mapped by hybridization experiments with genes from C. acetobutylicum and from various other clostridia as probes. The genetic map of C. acetobutylicum was compared to that of the three other endospore-forming bacteria characterized so far: Bacillus subtilis, Clostridium beijerinckii, and Clostridium perfringens. Paradoxically, the chromosomal backbone of C. acetobutylicum showed more similarity to that of B. subtilis than to those of the clostridia.