GRANULOCYTE COLONY-STIMULATING FACTOR IMPROVES ALTERNATIVE ACTIVATION OF MICROGLIA UNDER MICROENVIRONMENT OF SPINAL CORD INJURY
GRANULOCYTE COLONY-STIMULATING FACTOR IMPROVES ALTERNATIVE ACTIVATION OF MICROGLIA UNDER MICROENVIRONMENT OF SPINAL CORD INJURY
复制标题
粒细胞集落刺激因子改善脊髓损伤微环境下小胶质细胞的选择性激活。
DOI：
10.1016/j.neuroscience.2013.01.047
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发表时间：
2013-05-15
期刊：
影响因子：
3.3
通讯作者：
Hao, A.
中科院分区：
文献类型：
--
作者：
Guo, Y.;Zhang, H.;Hao, A.
本研究旨在探讨粒细胞集落刺激因子（G-CSF）对脊髓损伤微环境下小胶质细胞活化状态的影响，为脊髓损伤的治疗提供新的思路。建立小鼠脊髓半横断模型，皮下注射重组人粒细胞集落刺激因子（rhG-CSF）。结果表明，G-CSF可在脊髓损伤后72 h内将小胶质细胞募集到损伤部位。此外，G-CSF抑制促炎因子的表达，促进神经营养因子的表达。此外，G-CSF还能增加BV 2小胶质细胞M2巨噬细胞标志物的表达，抑制M1巨噬细胞标志物的表达，有利于脊髓半切微环境下小胶质细胞的M2极化。NF κ B B信号通路参与G-CSF诱导的BV 2小胶质细胞极化。总之，我们认为，在脊髓损伤后的第一个72小时内给予G-CSF可能会减少早期炎症诱导的有害影响，并通过改善小胶质细胞的替代激活促进有利于修复的抗炎反应。在脊髓损伤急性期给予G-CSF可能是脊髓损伤后恢复治疗的一个有前途的策略。(C)2013年IBRO。由Elsevier Ltd.出版。保留所有权利。
Granulocyte colony-stimulating factor (G-CSF) was investigated in the present study to examine whether it could affect the activation status of microglia under microenvironment of spinal cord injury and provide a potential therapeutic treatment for spinal cord injury. We established mouse spinal cord hemisection model and injected recombinant human G-CSF (rhG-CSF) subcutaneously. The results demonstrated that G-CSF could recruit microglia to the injury site in the first 72 h after spinal cord injury. Moreover, G-CSF inhibits the expression of pro-inflammatory factors and promotes the expression of neurotrophic factors. Additionally, G-CSF also increases the expression of markers of M2 macrophage and inhibits the expression of markers of M1 macrophage in BV2 microglia in vitro model, favoring the M2 polarization of microglia under the microenvironment of spinal cord hemisection. NF kappa B signal pathway was involved in G-CSF-induced polarization of BV2 microglia. As a conclusion, we suggested that administration of G-CSF within the first 72 h after spinal cord injury might reduce early inflammation-induced detrimental effect and promote an anti-inflammatory response that favors repair via improving alternative activation of microglia. Administration of G-CSF in the acute phase of spinal cord injury may be a promising strategy in restorative therapy after spinal cord injury. (C) 2013 IBRO. Published by Elsevier Ltd. All rights reserved.