MAF1, a novel plant protein interacting with matrix attachment region binding protein MFP1, is located at the nuclear envelope
MAF1, a novel plant protein interacting with matrix attachment region binding protein MFP1, is located at the nuclear envelope
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DOI：
10.1105/tpc.11.9.1755
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发表时间：
1999-09-01
期刊：
影响因子：
11.6
通讯作者：
Meier, I
中科院分区：
文献类型：
--
作者：
Gindullis, P;Peffer, NJ;Meier, I
染色质通过基质附着区(MAR)DNA与核基质的相互作用被认为是所有真核细胞中染色质组织的基础。番茄MAR结合丝状蛋白1(MFP1)是一种新型的植物蛋白，能与MAR DNA特异结合，其丝状蛋白结构使其有可能成为核基质的结构成分。MFP1位于核膜上与核基质相关的斑点状结构。在这里，我们报告了在酵母双杂交和体外结合试验中鉴定出一种新的与MFP 1特异相互作用的蛋白质。MFP1相关因子1(MAF1)是一种小分子可溶性丝氨酸/苏氨酸丰富的蛋白质，广泛表达，与已知蛋白质没有相似性。MAF1和MFP1一样，位于核的外围，是核基质的组成部分。这些数据表明，MFP1和MAF1是体内相互作用的伙伴，这两种蛋白都是以前在植物中未描述的核亚结构的组成部分，连接核膜和内部核基质。
The interaction of chromatin with the nuclear matrix via matrix attachment region (MAR) DNA is considered to be of fundamental importance for chromatin organization in all eukaryotic cells. MAR binding filament-like protein 1 (MFP1) from tomato is a novel plant protein that specifically binds to MAR DNA, Its filament protein-like structure makes it a likely candidate for a structural component of the nuclear matrix. MFP1 is located at nuclear matrix-associated, specklelike structures at the nuclear envelope. Here, we report the identification of a novel protein that specifically interacts with MFP1 in yeast two-hybrid and in vitro binding assays. MFP1 associated factor 1 (MAF1) is a small, soluble, serine/threonine-rich protein that is ubiquitously expressed and has no similarity to known proteins. MAF1, like MFP1, is located at the nuclear periphery and is a component of the nuclear matrix. These data suggest that MFP1 and MAF1 are in vivo interaction partners and that both proteins are components of a nuclear substructure, previously undescribed in plants, that connects the nuclear envelope anti the internal nuclear matrix.