Low-intensity pulsed ultrasound (LIPUS) induces RANKL, MCP-1, and MIP-1beta expression in osteoblasts through the angiotensin II type 1 receptor.
Low-intensity pulsed ultrasound (LIPUS) induces RANKL, MCP-1, and MIP-1beta expression in osteoblasts through the angiotensin II type 1 receptor.
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发表时间：
2007
影响因子：
5.6
通讯作者：
K. Bandow;Yoshiaki Nishikawa;T. Ohnishi;K. Kakimoto;K. Soejima;S. Iwabuchi;K. Kuroe;T. Matsuguchi-T.-Matsuguc
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文献类型：
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作者：
K. Bandow;Yoshiaki Nishikawa;T. Ohnishi;K. Kakimoto;K. Soejima;S. Iwabuchi;K. Kuroe;T. Matsuguchi-T.-Matsuguc
恒定的机械应力对于维持骨量和强度至关重要，这是通过成骨细胞和破骨细胞的协作功能实现的。然而，尚未完全阐明这些细胞类型如何介导机械信号。低强度脉冲超声（LIPUS）疗法是最近开发的一种应用机械应力的方法，在临床上用于促进骨折愈合。在本研究中，我们将LIPUS应用于不同成熟阶段的成骨细胞，并分析其趋化因子和细胞因子的表达。与未成熟的成骨细胞相比，经过LIPUS处理数小时后，成熟的成骨细胞表达核因子κB配体受体激活剂（RANKL）、单核细胞趋化蛋白（MCP）-1和巨噬细胞炎症蛋白（MIP）-1β的mRNA水平显着升高。有趣的是，在成骨细胞中检测到血管紧张素 II 1 型受体 (AT1)（心肌细胞中已知的机械感受器）的蛋白质和 mRNA 表达，并且表达水平在细胞成熟过程中显着增加。此外，LIPUS 诱导的细胞外信号调节激酶 (ERK) 磷酸化和 RANKL/趋化因子表达被特定的 AT1 抑制剂消除。因此，AT1可能作为成骨细胞的机械感受器在骨代谢中发挥重要作用之一。
Constant mechanical stress is essential for the maintenance of bone mass and strength, which is achieved through the cooperative functions of osteoblasts and osteoclasts. However, it has not been fully elucidated how these cell types mediate mechanical signals. Low-intensity pulsed ultrasound (LIPUS) therapy is a recently developed method for application of mechanical stress, and is used clinically to promote bone fracture healing. In the present study, we applied LIPUS to osteoblasts at different stages of maturation and analyzed their chemokine and cytokine expression. In comparison with their immature counterparts, mature osteoblasts expressed significantly higher levels of mRNAs for the receptor activator of nuclear factor kappa B ligand (RANKL), monocyte chemoattractant protein (MCP)-1, and macrophage-inflammatory protein (MIP)-1beta after a few hours of LIPUS treatment. Intriguingly, protein and mRNA expression of angiotensin II type 1 receptor (AT1), a known mechanoreceptor in cardiomyocytes, was detected in osteoblasts, and the level of expression increased significantly during cell maturation. Furthermore, LIPUS-induced extracellular signal-regulated kinase (ERK) phosphorylation and RANKL/chemokine expression was abrogated by a specific AT1 inhibitor. Thus, AT1 may play one of the essential roles in bone metabolism as a mechanoreceptor of osteoblasts.