Expression of Sp7 in Satb2-induced osteogenic differentiation of mouse bone marrow stromal cells is regulated by microRNA-27a
Expression of Sp7 in Satb2-induced osteogenic differentiation of mouse bone marrow stromal cells is regulated by microRNA-27a
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DOI：
10.1007/s11010-016-2709-y
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发表时间：
2016-06-01
影响因子：
4.3
通讯作者：
Yu, Youcheng
中科院分区：
文献类型：
--
作者：
Gong, Yiming;Lu, Jing;Yu, Youcheng
Satb2是一种富含AT的特异性结合转录因子，对成骨细胞分化和骨形成至关重要。特定的微小RNA（miRNAs）已被确定可调节成骨分化的复杂过程。在Satb2诱导的骨髓基质细胞（BMSCs）成骨分化过程中，miRNA的表达如何变化仍不清楚。从我们收集的Satb2诱导小鼠BMSCs成骨分化的miRNA表达谱数据中，我们发现诱导后7天，相对于未处理的细胞，miR - 27a显著下调。通过计算机分析，我们确定Sp7为miR - 27a的靶基因，并通过蛋白质印迹分析、定量逆转录聚合酶链反应（qRT - PCR）和荧光素酶报告基因检测验证了这一发现。我们还通过将外源性miR - 27a转染到BMSCs中分析了miR - 27a在成骨分化中的功能。miR - 27a的过表达显著抑制了Sp7的表达，并减弱了Satb2诱导的成骨分化。我们的结果表明，在Satb2诱导BMSCs成骨分化的早期阶段，Sp7的表达受miR - 27a调控。
Satb2 is a special AT-rich binding transcription factor essential for osteoblast differentiation and bone formation. Specific microRNAs (miRNAs) have been identified to regulate the complex process of osteogenic differentiation. It remains unclear how miRNA expressions is changed in the Satb2-induced osteogenic differentiation of bone marrow stromal cells (BMSCs). From the miRNA expression profile data collected by us from Satb2-induced osteogenic differentiation of mouse BMSCs, we found that miR-27a was significantly down-regulated relative to non-treated cells 7 days post induction. By in silico analysis, we identified Sp7 as a miR-27a targeting gene and verified the findings by Western blot analysis, qRT-PCR, and luciferase reporter assays. We also analyzed the function of miR-27a in osteogenic differentiation by transfection of exogenous miR-27a into BMSCs. Overexpression of miR-27a remarkably inhibited the expression of Sp7 and attenuated Satb2-induced osteogenic differentiation. Our results suggest that expression of Sp7 during the early stage of Satb2-induced osteogenic differentiation of BMSCs is regulated by miR-27a.